Structural basis of TraD and sbmA recognition by TraM of F-like plasmids

JJW Wong - 2012 - era.library.ualberta.ca
Bacterial conjugation is the process of plasmid DNA transfer from a donor cell to a recipient
cell. This process is mediated in F-like plasmids by proteins expressed by the tra operon …

Structural basis of specific TraD–TraM recognition during F plasmid‐mediated bacterial conjugation

J Lu, JJW Wong, RA Edwards, J Manchak… - Molecular …, 2008 - Wiley Online Library
F plasmid‐mediated bacterial conjugation requires interactions between a relaxosome
component, TraM, and the coupling protein TraD, a hexameric ring ATPase that forms the …

Relaxosome function and conjugation regulation in F‐like plasmids–a structural biology perspective

JJW Wong, J Lu, JNM Glover - Molecular microbiology, 2012 - Wiley Online Library
The tra operon of the prototypical F plasmid and its relatives enables transfer of a copy of the
plasmid to other bacterial cells via the process of conjugation. Tra proteins assemble to form …

The F plasmid‐encoded TraM protein stimulates relaxosome‐mediated cleavage at oriT through an interaction with TraI

H Ragonese, D Haisch, E Villareal… - Molecular …, 2007 - Wiley Online Library
Conjugative DNA transfer is a highly conserved process for the direct transfer of DNA from a
donor to a recipient. The conjugative initiator proteins are key players in the DNA processing …

Insights into the regulation of F and ColE1 plasmid transfer

BJ Pedrycz - 2014 - era.library.ualberta.ca
Multidrug resistant bacteria pose a threat to human health and economic burden for the
healthcare system. Transmission of antibiotic resistance genes can occur by the conjugative …

[图书][B] The role of F plasmid relaxosome protein binding sites and intervening origin of transfer (oriT) regions in bacterial conjugation

SL Williams - 2008 - search.proquest.com
Bacterial conjugation is the transfer of single-stranded DNA (ssDNA) from one bacterium to
another. The initiation of transfer requires a complex of proteins called the relaxosome. The …

Mutations in the C-terminal region of TraM provide evidence for in vivo TraM-TraD interactions during F-plasmid conjugation

J Lu, LS Frost - Journal of bacteriology, 2005 - Am Soc Microbiol
Conjugation is a major mechanism for disseminating genetic information in bacterial
populations, but the signal that triggers it is poorly understood in gram-negative bacteria. F …

Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM

JJW Wong, J Lu, RA Edwards, LS Frost… - Nucleic acids …, 2011 - academic.oup.com
The conjugative transfer of F-like plasmids such as F, R1, R100 and pED208, between
bacterial cells requires TraM, a plasmid-encoded DNA-binding protein. TraM tetramers …

[PDF][PDF] Characterizing the protein and DNA interactions of the F plasmid DNA binding protein, TraM

RA Fekete - 2001 - era.library.ualberta.ca
Many host-and plasmid-encoded proteins are required to efficiently transfer the Escherichia
coli F plasmid. The latter are divided into five functional groups: pilus synthesis and …

Thirty-eight C-terminal amino acids of the coupling protein TraD of the F-like conjugative resistance plasmid R1 are required and sufficient to confer binding to the …

A Beranek, M Zettl, K Lorenzoni, A Schauer… - Journal of …, 2004 - Am Soc Microbiol
Coupling proteins (CPs) are present in type IV secretion systems of plant, animal, and
human pathogens and are essential for DNA transfer in bacterial conjugation systems. CPs …