Effect of external cryoprotectants as membrane stabilizers on cryopreserved rainbow trout sperm

E Cabrita, L Anel, MP Herraez - Theriogenology, 2001 - Elsevier
The process of freezing and thawing induces certain cellular damage in rainbow trout
(Oncorhynchus mykiss) spermatozoa. We have previously demonstrated that after freezing …

Sublethal damage during cryopreservation of rainbow trout sperm

E Cabrita, R Alvarez, L Anel, KJ Rana, MP Herraez - Cryobiology, 1998 - Elsevier
Although cellular damage during cryopreservation of freshwater fish spermatozoa has been
reported in several studies, there is a lack of correlation between this damage and the …

[PDF][PDF] Sperm cryopreservation in fish and shellfish

TR Tiersch, H Yang, JA Jenkins… - Society of Reproduction …, 2007 - researchgate.net
The scientific underpinnings of cryobiology can be traced back to the 1950s after the
discovery of the cryoprotective qualities of glycerol for fowl sperm (Polge, Smith and Parkes …

Effects of various cryoprotective agents and membrane-stabilizing compounds on bull sperm membrane integrity after cooling and freezing

FE De Leeuw, AM De Leeuw, JHG Den Daas… - Cryobiology, 1993 - Elsevier
In this study attempts were made to improve the survival rates of bull spermatozoa after
freezing/thawing and to clarify the importance of certain agents to the cryopreservation of …

Effects of temperature and restoration of osmotic equilibrium during thawing on the induction of plasma membrane damage in cryopreserved ram spermatozoa

WV Holt, RD North - Biology of Reproduction, 1994 - academic.oup.com
The objective of this investigation was to examine the nature of freeze/thaw-induced plasma
membrane damage in an effort to validate hypotheses about cryoinjury in ram spermatozoa …

Cryopreservation of rainbow trout (Oncorhynchus mykiss) spermatozoa using programmable freezing

P Conget, M Fernández, G Herrera, JJ Minguell - Aquaculture, 1996 - Elsevier
In an attempt to establish a protocol for the cryopreservation of the spermatozoa of the
rainbow trout (Oncorhynchus mykiss), we studied the effect of various cryoprotective agents …

Molecular and subcellular cryoinjury of fish spermatozoa and approaches to improve cryopreservation

M Xin, H Niksirat, A Shaliutina‐Kolešová… - Reviews in …, 2020 - Wiley Online Library
The quality of frozen/thawed fish sperm is generally lower than that of fresh sperm.
Extremely low temperatures are associated with damage to fish spermatozoon subcellular …

Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology

E Figueroa, I Valdebenito, O Merino… - Journal of fish …, 2016 - Wiley Online Library
The objective of this study was to determine the effect of freezing on the function in Atlantic
salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium+ 1. 3M …

Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: stability of mitochondrion as criterion of effectiveness

O Merino, J Risopatron, R Sanchez, E Isachenko… - Animal reproduction …, 2011 - Elsevier
The aim of the present investigations was to test a novel technology comprising
cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of …

Influence of rainbow trout thermal acclimation on sperm cryopreservation: relation to change in the lipid composition of the plasma membrane

C Labbé, G Maisse - Aquaculture, 1996 - Elsevier
The ability of rainbow trout (Oncorhynchus mykiss) spermatozoa to withstand
cryopreservation was tested after the fish and sperm were exposed to various temperature …