Obtaining meaningful snapshots of the metabolome of microorganisms requires rapid
sampling and immediate quenching of all metabolic activity, to prevent any changes in …

Sample preparation is one of the most important steps in metabolome analysis. The
challenges of determining microbial metabolome have been well discussed within the …

Targeted metabolomics, aimed at the study of metabolic reaction networks and their
regulation in vivo, is a rapidly emerging field in systems biology. Obtaining proper …

Bacteria are remarkably diverse in terms of their size, structure and biochemical properties.
Due to this fact, it is hard to develop a universal method for handling bacteria cultures during …

Metabolomics is a comprehensive analysis of metabolites existing in biological systems. As
one of the important “omics” tools, the approach has been widely employed in various fields …

Targeted metabolomics aims at reliable and sensitive quantification of a subset of
metabolites in a cell. Here, we describe our current methods to obtain quantitative …

Microbial metabolomics has received much attention in recent years mainly because it
supports and complements a wide range of microbial research areas from new drug …

In the present work we investigated the most commonly applied methods used for sampling
of microorganisms in the field of metabolomics in order to unravel potential sources of error …

The reproducibility and accuracy of analysis methods has expanded rapidly over the past
years. Hyphenated methods, for example, GC-MS and LC-MS/MS (liquid chromatography …

Because of the importance of microbes as model organisms, biotechnology tools, and
contributors to mammalian and ecosystem metabolism, there has been longstanding interest …