Development of a gene expression vector for Thermus thermophilus based on the promoter of the respiratory nitrate reductase
A specific expression system for Thermus spp. is described. Plasmid pMKE1 contains
replicative origins for Escherichia coli and Thermus spp., a selection gene encoding a …
replicative origins for Escherichia coli and Thermus spp., a selection gene encoding a …
A thermophilic nitrate reductase is responsible for the strain specific anaerobic growth of Thermus thermophilus HB8
S Ramı́rez-Arcos, LA Fernández-Herrero… - … et Biophysica Acta (BBA …, 1998 - Elsevier
T. thermophilus HB8 contains a nitrate reductase gene cluster which is absent from closely
related strains. This cluster encodes 4 ORFs (a–d) similar in organization and protein …
related strains. This cluster encodes 4 ORFs (a–d) similar in organization and protein …
Transferable denitrification capability of Thermus thermophilus
Laboratory-adapted strains of Thermus spp. have been shown to require oxygen for growth,
including the model strains T. thermophilus HB27 and HB8. In contrast, many isolates of this …
including the model strains T. thermophilus HB27 and HB8. In contrast, many isolates of this …
Thermus thermophilus as a cell factory for the production of a thermophilic Mn-dependent catalase which fails to be synthesized in an active form in Escherichia coli
Thermostable Mn-dependent catalases are promising enzymes in biotechnological
applications as H2O2-detoxifying systems. We cloned the genes encoding Mn-dependent …
applications as H2O2-detoxifying systems. We cloned the genes encoding Mn-dependent …
Lateral Transfer of the Denitrification Pathway Genes among Thermus thermophilus Strains
L Alvarez, C Bricio, M Jose Gomez… - Applied and …, 2011 - Am Soc Microbiol
Nitrate respiration is a common and strain-specific property in Thermus thermophilus
encoded by the nitrate respiration conjugative element (NCE) that can be laterally …
encoded by the nitrate respiration conjugative element (NCE) that can be laterally …
Biochemical and regulatory properties of a respiratory island encoded by a conjugative plasmid in the extreme thermophile Thermus thermophilus
F Cava, J Berenguer - 2006 - portlandpress.com
In the present paper, we summarize the current knowledge on the first step of the
denitrification pathway in the ancestral extreme thermophilic bacterium Thermus …
denitrification pathway in the ancestral extreme thermophilic bacterium Thermus …
Anaerobic growth, a property horizontally transferred by an Hfr-like mechanism among extreme thermophiles
S Ramírez-Arcos, LA Fernández-Herrero… - Journal of …, 1998 - Am Soc Microbiol
Despite the fact that the extreme thermophilic bacteria belonging to the genus Thermus are
classified as strict aerobes, we have shown that Thermus thermophilus HB8 (ATCC 27634) …
classified as strict aerobes, we have shown that Thermus thermophilus HB8 (ATCC 27634) …
Two Nitrate/Nitrite Transporters Are Encoded within the Mobilizable Plasmid for Nitrate Respiration of Thermus thermophilus HB8
Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate
reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In …
reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In …
Control of the respiratory metabolism of Thermus thermophilus by the nitrate respiration conjugative element NCE
F Cava, O Laptenko, S Borukhov… - Molecular …, 2007 - Wiley Online Library
The strains of Thermus thermophilus that contain the n itrate respiration c onjugative e
lement (NCE) replace their aerobic respiratory chain by an anaerobic counterpart made of …
lement (NCE) replace their aerobic respiratory chain by an anaerobic counterpart made of …
A high-transformation-efficiency cloning vector for Thermus thermophilus
M de Grado, P Castán, J Berenguer - Plasmid, 1999 - Elsevier
The cloning vector pMK18 was developed through the fusion of the minimal replicative
region from an indigenous plasmid of Thermus sp. ATCC27737, a gene cassette encoding a …
region from an indigenous plasmid of Thermus sp. ATCC27737, a gene cassette encoding a …