Development of a gene expression vector for Thermus thermophilus based on the promoter of the respiratory nitrate reductase

R Moreno, O Zafra, F Cava, J Berenguer - Plasmid, 2003 - Elsevier
A specific expression system for Thermus spp. is described. Plasmid pMKE1 contains
replicative origins for Escherichia coli and Thermus spp., a selection gene encoding a …

A thermophilic nitrate reductase is responsible for the strain specific anaerobic growth of Thermus thermophilus HB8

S Ramı́rez-Arcos, LA Fernández-Herrero… - … et Biophysica Acta (BBA …, 1998 - Elsevier
T. thermophilus HB8 contains a nitrate reductase gene cluster which is absent from closely
related strains. This cluster encodes 4 ORFs (a–d) similar in organization and protein …

Transferable denitrification capability of Thermus thermophilus

L Alvarez, C Bricio, A Blesa, A Hidalgo… - Applied and …, 2014 - Am Soc Microbiol
Laboratory-adapted strains of Thermus spp. have been shown to require oxygen for growth,
including the model strains T. thermophilus HB27 and HB8. In contrast, many isolates of this …

Thermus thermophilus as a cell factory for the production of a thermophilic Mn-dependent catalase which fails to be synthesized in an active form in Escherichia coli

A Hidalgo, L Betancor, R Moreno, O Zafra… - Applied and …, 2004 - Am Soc Microbiol
Thermostable Mn-dependent catalases are promising enzymes in biotechnological
applications as H2O2-detoxifying systems. We cloned the genes encoding Mn-dependent …

Lateral Transfer of the Denitrification Pathway Genes among Thermus thermophilus Strains

L Alvarez, C Bricio, M Jose Gomez… - Applied and …, 2011 - Am Soc Microbiol
Nitrate respiration is a common and strain-specific property in Thermus thermophilus
encoded by the nitrate respiration conjugative element (NCE) that can be laterally …

Biochemical and regulatory properties of a respiratory island encoded by a conjugative plasmid in the extreme thermophile Thermus thermophilus

F Cava, J Berenguer - 2006 - portlandpress.com
In the present paper, we summarize the current knowledge on the first step of the
denitrification pathway in the ancestral extreme thermophilic bacterium Thermus …

Anaerobic growth, a property horizontally transferred by an Hfr-like mechanism among extreme thermophiles

S Ramírez-Arcos, LA Fernández-Herrero… - Journal of …, 1998 - Am Soc Microbiol
Despite the fact that the extreme thermophilic bacteria belonging to the genus Thermus are
classified as strict aerobes, we have shown that Thermus thermophilus HB8 (ATCC 27634) …

Two Nitrate/Nitrite Transporters Are Encoded within the Mobilizable Plasmid for Nitrate Respiration of Thermus thermophilus HB8

S Ramírez, R Moreno, O Zafra, P Castán… - Journal of …, 2000 - Am Soc Microbiol
Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate
reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In …

Control of the respiratory metabolism of Thermus thermophilus by the nitrate respiration conjugative element NCE

F Cava, O Laptenko, S Borukhov… - Molecular …, 2007 - Wiley Online Library
The strains of Thermus thermophilus that contain the n itrate respiration c onjugative e
lement (NCE) replace their aerobic respiratory chain by an anaerobic counterpart made of …

A high-transformation-efficiency cloning vector for Thermus thermophilus

M de Grado, P Castán, J Berenguer - Plasmid, 1999 - Elsevier
The cloning vector pMK18 was developed through the fusion of the minimal replicative
region from an indigenous plasmid of Thermus sp. ATCC27737, a gene cassette encoding a …