An experimental methodology for measuring volume changes in proton transfer reactions in aqueous solutions

C Viappiani, S Abbruzzetti, JR Small, LJ Libertini… - Biophysical …, 1998 - Elsevier
A fast perturbation in proton concentration can be induced in aqueous solution using a
pulsed ultraviolet laser and suitable photolabile compounds which, upon photoexcitation …

Study of proton transfer processes in solution using the laser induced pH-jump: a new experimental setup and an improved data analysis based on genetic algorithms

C Viappiani, G Bonetti, M Carcelli, F Ferrari… - Review of scientific …, 1998 - pubs.aip.org
We present an experimental setup for studying proton transfer reactions in solution and an
innovative data analysis based on a genetic algorithm. We have used o-nitrobenzaldehyde …

The pH Jump: Probing of Macromolecules and Solutions by a Laser‐Induced, Ultrashort Proton Pulse—Theory and Applications in Biochemistry

M Gutman - Methods of biochemical analysis, 1984 - Wiley Online Library
The pH Jump: Probing of Macromolecules and Solutions by a Laser‐Induced, Ultrashort Proton
Pulse—Theory and Applications in Biochemistry Page 8 METHODS OF BIOCHEMICAL ANALYSIS …

Reaction volume of water formation detected by time-resolved photoacoustics: photoinduced proton transfer between o-nitrobenzaldehyde and hydroxyls in water

G Bonetti, A Vecli, C Viappiani - Chemical physics letters, 1997 - Elsevier
The structural volume changes accompanying light-induced proton transfer reactions in
aqueous solutions of o-nitrobenzaldehyde have been investigated using time-resolved …

[39] Application of the laser-induced proton pulse for measuring the protonation rate constants of specific sites on proteins and membranes

M Gutman - Methods in Enzymology, 1986 - Elsevier
Publisher Summary This chapter describes a technique of laser-induced proton pulse that is
capable of measuring the protonation rate constants of specific sites on proteins and …

Primary ultrafast events preceding the photoinduced proton transfer from pyranine to water

TH Tran-Thi, T Gustavsson, C Prayer, S Pommeret… - Chemical Physics …, 2000 - Elsevier
Femtosecond fluorescence and absorption spectroscopies are used to probe the early
events of the photoinduced proton transfer (PT) from pyranine to water. The process is found …

[HTML][HTML] Kinetics of local helix formation in poly-L-glutamic acid studied by time-resolved photoacoustics: neutralization reactions of carboxylates in aqueous solutions …

S Abbruzzetti, C Viappiani, JR Small, LJ Libertini… - Biophysical …, 2000 - cell.com
Photoactivatable caged protons have been used to trigger proton transfer reactions in
aqueous solutions of acetate, glutamate, and poly-L-glutamic acid, and the volumetric and …

Kinetics of proton transfer in a green fluorescent protein: a laser-induced pH jump study

R Mallik, JB Udgaonkar, G Krishnamoorthy - Journal of Chemical …, 2003 - Springer
The sub-millisecond protonation dynamics of the chromophore in S65T mutant form of the
green fluorescent protein (GFP) was tracked after a rapid pH jump following laser-induced …

Dynamic studies of proton diffusion in mesoscopic heterogeneous matrix: I. Concentrated solutions of sucrose

M Gutman, E Nachliel, S Kiryati - Biophysical journal, 1992 - cell.com
Biochemical systems lose their homogeneity at a mesoscopic scale; physical parameters
vary sharply over a scale of a few nanometers. In this manuscript, we demonstrate how …

The pH jump: kinetic analysis and determination of the diffusion-controlled rate constants

M Gutman, E Nachliel, E Gershon… - Journal of the …, 1983 - ACS Publications
Abstract; Excitation of proton-emitter compounds such as 8-hydroxypyrene-1, 3, 6-
trisulfonate or sulfonated/3-naphthol derivatives by short, intensive laser pulses discharges …