Setting up an ancient DNA laboratory

TL Fulton - Ancient DNA: methods and protocols, 2012 - Springer
Entering into the world of ancient DNA research is nontrivial. Because the DNA in most
ancient specimens is degraded to some extent, the potential for contamination of ancient …

Setting up an ancient DNA laboratory

TL Fulton, B Shapiro - Ancient DNA: methods and protocols, 2019 - Springer
Entering into the world of ancient DNA research is nontrivial. Because the DNA in most
ancient specimens is degraded to some extent, the potential is high for contamination of …

From the field to the laboratory: Controlling DNA contamination in human ancient DNA research in the high-throughput sequencing era

B Llamas, G Valverde, L Fehren-Schmitz… - STAR: Science & …, 2017 - Taylor & Francis
Abstract High-Throughput DNA Sequencing (HTS) technologies have changed the way in
which we detect and assess DNA contamination in ancient DNA studies. Researchers use …

Contamination controls when preparing archaeological remains for ancient DNA analysis

DY Yang, K Watt - Journal of Archaeological Science, 2005 - Elsevier
Contamination is of utmost concern when working with ancient DNA as it easily leads to
false positive results. The best way to prevent or minimize contamination is to start …

Setting the stage–Building and working in an ancient DNA laboratory

M Knapp, AC Clarke, KA Horsburgh… - Annals of Anatomy …, 2012 - Elsevier
With the introduction of next generation high throughput sequencing in 2005 and the
resulting revolution in genetics, ancient DNA research has rapidly developed from an …

Ancient DNA is thirteen years old

S Audio, E Béraud-Colomb - Nature biotechnology, 1997 - nature.com
The first successful recovery of ancient DNA, from quagga and human mummies inspired
significant enough interest to open an entire field of research. Efforts from many research …

Partial uracil–DNA–glycosylase treatment for screening of ancient DNA

N Rohland, E Harney, S Mallick… - … Transactions of the …, 2015 - royalsocietypublishing.org
The challenge of sequencing ancient DNA has led to the development of specialized
laboratory protocols that have focused on reducing contamination and maximizing the …

Ancient DNA: do it right or not at all

HN Poinar, A Cooper - Science, 2000 - search.proquest.com
At the recent 5th International Ancient DNA Conference in Manchester, UK, re-ported by Erik
Stokstad in his News Focus article “Divining diet and disease from DN”(28 Jul., p. 530), one …

New uses for old DNA

A Cooper, R Wayne - Current Opinion in Biotechnology, 1998 - Elsevier
Several years have elapsed since the last report of million-year-old DNA, coinciding with
increased standards for experimental procedures in ancient DNA research. Whereas many …

Comparison and optimization of ancient DNA extraction

N Rohland, M Hofreiter - Biotechniques, 2007 - Taylor & Francis
Ancient DNA analyses rely on the extraction of the tiny amounts of DNA remaining in
samples that are hundreds to tens of thousands of years old. Despite the critical role …