Primase is an essential DNA replication enzyme in Escherichia coli and responsible for
primer synthesis during lagging strand DNA replication. Although the interaction of primase …

Revised Manuscript Received September 29, 1995s abstract: The kinetics of primer RNA
initiation and elongation by Escherichia coli primase were measured. The single-stranded …

Escherichia coli primase/SSB/single-stranded phage G4oric is a simple system to study how
primase interacts with DNA template to synthesize primer RNA for initiation of DNA …

We find that neither the hairpins nor bound single-stranded DNA-binding protein (SSB) are
required for in vitro primase activity at the G4 complementary strand origin. Primase has the …

DNA primases catalyze the synthesis of the oligoribonucleotides required for the initiation of
lagging strand DNA synthesis. Biochemical studies have elucidated the mechanism for the …

Cysteine 39 of Escherichia coli primase is the most chemically reactive cysteine. Its high
chemical reactivity is likely due to its proximity to primase's zinc, which is probably ligated to …

The effect of DnaB helicase on the initiation specificity of primase was studied biochemically
using a series of single-stranded DNA templates in which each nucleotide of the …

Initiation and synthesis of RNA primers in the lagging strand of the replication fork in
Escherichia coli requires the replicative DnaB helicase and the DNA primase, the DnaG …

Primase plays a key role in governing the sequence of events required on the lagging strand
during a cycle of Okazaki fragment synthesis. To begin to probe the protein-protein …

The study of primases from model organisms such as Escherichia coli, phage T7 and phage
T4 has demonstrated the essential nature of primase function, which is to generate de novo …