PhenoGMM: Gaussian mixture modelling of microbial cytometry data enables efficient predictions of biodiversity

P Rubbens, R Props, FM Kerckhof, N Boon… - BioRxiv, 2019 - biorxiv.org
Microbial flow cytometry allows to rapidly characterize microbial communities. Recent
research has demonstrated a moderate to strong connection between the cytometric …

PhenoGMM: Gaussian mixture modeling of cytometry data quantifies changes in microbial community structure

P Rubbens, R Props, FM Kerckhof, N Boon… - MSphere, 2021 - Am Soc Microbiol
Microbial flow cytometry can rapidly characterize the status of microbial communities. Upon
measurement, large amounts of quantitative single-cell data are generated, which need to …

Predicting the presence and abundance of bacterial taxa in environmental communities through flow cytometric fingerprinting

J Heyse, F Schattenberg, P Rubbens, S Müller… - Msystems, 2021 - Am Soc Microbiol
Microbiome management research and applications rely on temporally resolved
measurements of community composition. Current technologies to assess community …

Measuring the biodiversity of microbial communities by flow cytometry

R Props, P Monsieurs, M Mysara… - Methods in Ecology …, 2016 - Wiley Online Library
Measuring the microbial diversity in natural and engineered environments is important for
ecosystem characterization, ecosystem monitoring and hypothesis testing. Although the …

[HTML][HTML] Recent advances in microbial community analysis from machine learning of multiparametric flow cytometry data

BDÖ Duygan, JR van der Meer - Current Opinion in Biotechnology, 2022 - Elsevier
Highlights•Large multiparametric flow cytometry data sets are ideal for machine learning.•
Unsupervised clustering methods highlight microbial community changes.•Supervised …

Computational analysis of microbial flow cytometry data

P Rubbens, R Props - MSystems, 2021 - Am Soc Microbiol
Flow cytometry is an important technology for the study of microbial communities. It grants
the ability to rapidly generate phenotypic single-cell data that are both quantitative …

Rapid detection of microbiota cell type diversity using machine-learned classification of flow cytometry data

BD Özel Duygan, N Hadadi, AF Babu… - Communications …, 2020 - nature.com
The study of complex microbial communities typically entails high-throughput sequencing
and downstream bioinformatics analyses. Here we expand and accelerate microbiota …

Microbiome meets classical microbiology: quantifying sample CFU using 16S rRNA gene sequencing data

GNF Cruz, AP Christoff, LFV de Oliveira - 2020 - researchsquare.com
Background Next-generation sequencing (NGS) has been extensively employed to perform
microbiome characterization worldwide. As a culture-independent methodology, it has …

[PDF][PDF] Development of an Automated Online Flow Cytometry Method to Quantify Cell Density and Fingerprint Bacterial Communities. Cells 2023, 12, 1559

J López-Gálvez, K Schiessl, MD Besmer, C Bruckmann… - 2023 - promicon.eu
Cell density is an important factor in all microbiome research, where interactions are of
interest. It is also the most important parameter for the operation and control of most …

flowEMMi: an automated model-based clustering tool for microbial cytometric data

J Ludwig, CH Zu Siederdissen, Z Liu, PF Stadler… - BMC …, 2019 - Springer
Background Flow cytometry (FCM) is a powerful single-cell based measurement method to
ascertain multidimensional optical properties of millions of cells. FCM is widely used in …