Technical recommendations to perform the alkaline standard and enzyme-modified comet assay in human biomonitoring studies

A Azqueta, D Muruzabal, E Boutet-Robinet… - … /Genetic Toxicology and …, 2019 - Elsevier
The comet assay (single cell gel electrophoresis) is widely used as a biomonitoring tool to
assess DNA damage–strand breaks, as well as oxidised bases; it can also be adapted to …

The essential comet assay: a comprehensive guide to measuring DNA damage and repair

A Azqueta, AR Collins - Archives of toxicology, 2013 - Springer
The comet assay (single cell gel electrophoresis) is the most common method for measuring
DNA damage in eukaryotic cells or disaggregated tissues. The assay depends on the …

Alkaline comet assay for assessing DNA damage in individual cells

X Pu, Z Wang, JE Klaunig - Current protocols in toxicology, 2015 - Wiley Online Library
Single‐cell gel electrophoresis, commonly called a comet assay, is a simple and sensitive
method for assessing DNA damage at the single‐cell level. It is an important technique in …

Towards a more reliable comet assay: optimising agarose concentration, unwinding time and electrophoresis conditions

A Azqueta, KB Gutzkow, G Brunborg… - … /Genetic Toxicology and …, 2011 - Elsevier
The comet assay is now the method of choice for measuring most kinds of DNA damage in
cells. However, due to the lack of a standardised protocol inter-laboratory comparisons are …

The comet assay: a sensitive genotoxicity test for the detection of DNA damage and repair

G Speit, A Rothfuss - DNA Repair Protocols, 2012 - Springer
The comet assay (single-cell gel electrophoresis) is a simple and sensitive method for
studying DNA damage and repair. In this microgel electrophoresis technique, a small …

The next three decades of the comet assay: a report of the 11th International Comet Assay Workshop

G Koppen, A Azqueta, B Pourrut, G Brunborg… - …, 2017 - academic.oup.com
Abstract The International Comet Assay Workshops are a series of scientific conferences
dealing with practical and theoretical aspects of the Comet Assay (single-cell gel …

Environmental and occupational biomonitoring using the Comet assay

M Valverde, E Rojas - Mutation Research/Reviews in Mutation Research, 2009 - Elsevier
Biomonitoring of human populations exposed to potential mutagens or carcinogens can
provide an early detection system for the initiation of cell disregulation in the development of …

Validation and implementation of an internal standard in comet assay analysis

M De Boeck, N Touil, G De Visscher, PA Vande… - … /Genetic Toxicology and …, 2000 - Elsevier
The comet assay is widely used to detect DNA damage in single cells. However, only
moderate attention has been paid to the experimental variability of this assay, especially …

The use of the alkaline comet assay with lymphocytes in human biomonitoring studies

F Faust, F Kassie, S Knasmüller, RH Boedecker… - … Research/Reviews in …, 2004 - Elsevier
We reviewed the data of 45 alkaline comet assay studies with lymphocytes published during
the last three years with the objective of monitoring human exposure to genotoxic agents as …

The comet assay in human biomonitoring

D Anderson, A Dhawan, J Laubenthal - Genotoxicity assessment: Methods …, 2013 - Springer
Human biomonitoring studies aim to identify potential exposures to environmental,
occupational, or lifestyle toxicants in human populations and are commonly used by public …