Restriction site‐associated DNA sequencing, genotyping error estimation and de novo assembly optimization for population genetic inference

A Mastretta‐Yanes, N Arrigo, N Alvarez… - Molecular ecology …, 2015 - Wiley Online Library
Restriction site‐associated DNA sequencing (RAD seq) provides researchers with the ability
to record genetic polymorphism across thousands of loci for nonmodel organisms …

[HTML][HTML] Selecting RAD-Seq data analysis parameters for population genetics: the more the better?

N Díaz-Arce, N Rodríguez-Ezpeleta - Frontiers in genetics, 2019 - frontiersin.org
Restriction site-associated DNA sequencing (RAD-seq) has become a powerful and widely
used tool in molecular ecology studies as it allows to cost-effectively recover thousands of …

These aren't the loci you'e looking for: Principles of effective SNP filtering for molecular ecologists

SJ O'Leary, JB Puritz, SC Willis, CM Hollenbeck… - 2018 - Wiley Online Library
Sequencing reduced‐representation libraries of restriction site‐associated DNA (RAD seq)
to identify single nucleotide polymorphisms (SNP s) is quickly becoming a standard …

Finding the right coverage: the impact of coverage and sequence quality on single nucleotide polymorphism genotyping error rates

ED Fountain, JN Pauli, BN Reid… - Molecular Ecology …, 2016 - Wiley Online Library
Restriction‐enzyme‐based sequencing methods enable the genotyping of thousands of
single nucleotide polymorphism (SNP) loci in nonmodel organisms. However, in contrast to …

Estimating and accounting for genotyping errors in RAD‐seq experiments

L Bresadola, V Link, CA Buerkle… - Molecular Ecology …, 2020 - Wiley Online Library
In non‐model organisms, evolutionary questions are frequently addressed using reduced
representation sequencing techniques due to their low cost, ease of use, and because they …

Lost in parameter space: a road map for stacks

JR Paris, JR Stevens… - Methods in Ecology and …, 2017 - Wiley Online Library
Restriction site‐Associated DNA sequencing (RAD‐seq) has become a widely adopted
method for genotyping populations of model and non‐model organisms. Generating a …

Breaking RAD: An evaluation of the utility of restriction site‐associated DNA sequencing for genome scans of adaptation

DB Lowry, S Hoban, JL Kelley, KE Lotterhos, LK Reed… - 2017 - Wiley Online Library
Understanding how and why populations evolve is of fundamental importance to molecular
ecology. Restriction site‐associated DNA sequencing (RAD seq), a popular reduced …

RADseq provides unprecedented insights into molecular ecology and evolutionary genetics: comment on Breaking RAD by Lowry et al. (2016)

GJ McKinney, WA Larson, LW Seeb… - Molecular ecology …, 2017 - Wiley Online Library
In their recently corrected manuscript,“Breaking RAD: An evaluation of the utility of restriction
site associated DNA sequencing for genome scans of adaptation”, Lowry et al. argue that …

Genotyping‐in‐Thousands by sequencing (GT‐seq) panel development and application to minimally invasive DNA samples to support studies in molecular ecology

DA Schmidt, NR Campbell… - Molecular Ecology …, 2020 - Wiley Online Library
Minimally invasive sampling (MIS) is widespread in wildlife studies; however, its utility for
massively parallel DNA sequencing (MPS) is limited. Poor sample quality and contamination …

RAD cap: sequence capture of dual‐digest RAD seq libraries with identifiable duplicates and reduced missing data

SL Hoffberg, TJ Kieran, JM Catchen… - Molecular ecology …, 2016 - Wiley Online Library
Molecular ecologists seek to genotype hundreds to thousands of loci from hundreds to
thousands of individuals at minimal cost per sample. Current methods, such as restriction …