Efficient transformation and artificial miRNA gene silencing in Lemna minor

A Cantó‐Pastor, A Mollá‐Morales, E Ernst… - Plant …, 2015 - Wiley Online Library
Despite rapid doubling time, simple architecture and ease of metabolic labelling, a lack of
genetic tools in the L emnaceae (duckweed) has impeded the full implementation of this …

Crop improvement through tissue culture

LF De Filippis - Improvement of Crops in the Era of Climatic Changes …, 2013 - Springer
Techniques developed for plant tissue culture and micropropagation are important enabling
technologies that should help meet the demand for more efficient agricultural and …

Robust Transgene Expression from Bicistronic mRNA in the Green Alga Chlamydomonas reinhardtii

M Onishi, JR Pringle - G3: Genes, Genomes, Genetics, 2016 - academic.oup.com
The unicellular green alga Chlamydomonas reinhardtii is a model organism that provides an
opportunity to understand the evolution and functional biology of the lineage that includes …

[HTML][HTML] Leaf transformation for efficient random integration and targeted genome modification in maize and sorghum

N Wang, L Ryan, N Sardesai, E Wu, B Lenderts… - Nature Plants, 2023 - nature.com
Transformation in grass species has traditionally relied on immature embryos and has
therefore been limited to a few major Poaceae crops. Other transformation explants …

Rapid construction and screening of artificial microRNA systems in Chlamydomonas reinhardtii

J Hu, X Deng, N Shao, G Wang, K Huang - The Plant Journal, 2014 - Wiley Online Library
The unicellular green algae C hlamydomonas reinhardtii is a classic model for the study of
flagella/cilia and photosynthesis, and it has recently been exploited for producing …

Gene silencing by artificial microRNAs in Chlamydomonas

T Zhao, W Wang, X Bai, Y Qi - The Plant Journal, 2009 - Wiley Online Library
Chlamydomonas reinhardtii is a unicellular green alga. It is a model system for studying
functions of the chloroplast, basal body and flagella. The completion of the Chlamydomonas …

Genetic engineering of a Lemna isoleucine auxotroph

LV Nguyen, KM Cox, JS Ke, CG Peele, LF Dickey - Transgenic research, 2012 - Springer
Lemna, a member of the Lemnaceae or duckweed family, is a small aquatic plant that can
be quickly transformed to produce recombinant proteins in a contained and controlled …

Genome editing in diatoms: achievements and goals

PG Kroth, AM Bones, F Daboussi, MI Ferrante… - Plant cell reports, 2018 - Springer
Diatoms are major components of phytoplankton and play a key role in the ecology of
aquatic ecosystems. These algae are of great scientific importance for a wide variety of …

A versatile system for functional analysis of genes and micro RNA s in cotton

Z Gu, C Huang, F Li, X Zhou - Plant biotechnology journal, 2014 - Wiley Online Library
Cotton is an important economic crop worldwide. Due to its long growth cycle, large genome
size and recalcitrance to stable transformation, traditional methods for the analysis of gene …

Achieving plant genome editing while bypassing tissue culture

X Ji, B Yang, D Wang - Trends in plant science, 2020 - cell.com
Conventional genetic transformation is a huge obstacle to the efficient development and
application of plant genome-editing (GE) technologies. Recently, Maher et al. reported …