Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming
J Deng, R Shoemaker, B Xie, A Gore… - Nature …, 2009 - nature.com
Current DNA methylation assays are limited in the flexibility and efficiency of characterizing
a large number of genomic targets. We report a method to specifically capture an arbitrary …
a large number of genomic targets. We report a method to specifically capture an arbitrary …
Targeted and genome-scale strategies reveal gene-body methylation signatures in human cells
Studies of epigenetic modifications would benefit from improved methods for high-
throughput methylation profiling. We introduce two complementary approaches that use next …
throughput methylation profiling. We introduce two complementary approaches that use next …
Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity
We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to
accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells …
accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells …
Genome-scale DNA methylation maps of pluripotent and differentiated cells
DNA methylation is essential for normal development,, and has been implicated in many
pathologies including cancer,. Our knowledge about the genome-wide distribution of DNA …
pathologies including cancer,. Our knowledge about the genome-wide distribution of DNA …
Comparison of sequencing-based methods to profile DNA methylation and identification of monoallelic epigenetic modifications
Abstract Analysis of DNA methylation patterns relies increasingly on sequencing-based
profiling methods. The four most frequently used sequencing-based technologies are the …
profiling methods. The four most frequently used sequencing-based technologies are the …
Extended-representation bisulfite sequencing of gene regulatory elements in multiplexed samples and single cells
The biological roles of DNA methylation have been elucidated by profiling methods based
on whole-genome or reduced-representation bisulfite sequencing, but these approaches do …
on whole-genome or reduced-representation bisulfite sequencing, but these approaches do …
Profiling DNA methylome landscapes of mammalian cells with single-cell reduced-representation bisulfite sequencing
The heterogeneity of DNA methylation within a population of cells necessitates DNA
methylome profiling at single-cell resolution. Recently, we developed a single-cell reduced …
methylome profiling at single-cell resolution. Recently, we developed a single-cell reduced …
Human DNA methylomes at base resolution show widespread epigenomic differences
DNA cytosine methylation is a central epigenetic modification that has essential roles in
cellular processes including genome regulation, development and disease. Here we …
cellular processes including genome regulation, development and disease. Here we …
Whole-genome bisulfite sequencing with improved accuracy and cost
M Suzuki, W Liao, F Wos, AD Johnston… - Genome …, 2018 - genome.cshlp.org
DNA methylation patterns in the genome both reflect and help to mediate transcriptional
regulatory processes. The digital nature of DNA methylation, present or absent on each …
regulatory processes. The digital nature of DNA methylation, present or absent on each …
The dynamics of DNA methylation fidelity during mouse embryonic stem cell self-renewal and differentiation
The faithful transmission of DNA methylation patterns through cell divisions is essential for
the daughter cells to retain a proper cell identity. To achieve a comprehensive assessment …
the daughter cells to retain a proper cell identity. To achieve a comprehensive assessment …