Spread of recombinant DNA by roots and pollen of transgenic potato plants, identified by highly specific biomonitoring using natural transformation of an Acinetobacter …

J de Vries, M Heine, K Harms… - Applied and …, 2003 - Am Soc Microbiol
Transgenic potato plants with the nptII gene coding for neomycin phosphotransferase
(kanamycin resistance) as a selection marker were examined for the spread of recombinant …

The natural transformation of the soil bacteria Pseudomonas stutzeri and Acinetobacter sp. by transgenic plant DNA strictly depends on homologous sequences in …

J de Vries, P Meier… - FEMS Microbiology …, 2001 - academic.oup.com
The nptII+ gene present in the genome of transgenic potato plants transforms naturally
competent cells of the soil bacteria Pseudomonas stutzeri and Acinetobacter BD413 (both …

Evaluation of possible horizontal gene transfer from transgenic plants to the soil bacterium Acinetobacter calcoaceticus BD413

KM Nielsen, F Gebhard, K Smalla, AM Bones… - Theoretical and applied …, 1997 - Springer
The use of genetically engineered crop plants has raised concerns about the transfer of their
engineered DNA to indigenous microbes in soil. We have evaluated possible horizontal …

Detection of nptII (kanamycin resistance) genes in genomes of transgenic plants by marker-rescue transformation

J de Vries, W Wackernagel - Molecular and General Genetics MGG, 1998 - Springer
We have developed a novel system for the sensitive detection of nptII genes (kanamycin
resistance determinants) including those present in transgenic plant genomes. The assay is …

Monitoring the Spread of Recombinant DNA from Field Plots with Transgenic Sugar Beet Plants by PCR and Natural Transformation of Pseudomonas Stutzeri

P Meier, W Wackernagel - Transgenic research, 2003 - Springer
Previous studies had shown that recombinant DNA can be detected for several months in
soil after the deposition of litter from transgenic (tg) plants. Here we show by PCR monitoring …

Bacterial communities influenced by transgenic plants.

K Smalla, F Gebhard, JD Elsas, A Matzk, J Schiemann - 1994 - cabidigitallibrary.org
The persistence of transgenic Agrobacterium tumefaciens was investigated in field releases
of transgenic sugarbeet and potato plants in Germany in 1993 and 1994. Leaf material …

Detection of potential transgenic plant DNA recipients among soil bacteria

JM Monier, D Bernillon, E Kay, A Faugier… - Environmental …, 2007 - cambridge.org
The likelihood of gene transfer from transgenic plants to bacteria is dependent on gene
number and the presence of homologous sequences. The large number of transgene copies …

Agrobacterium-mediated transformation of New Zealand potato cultivars

AJ Conner, MK Williams, RC Gardner… - New Zealand Journal …, 1992 - Taylor & Francis
An Agrobacterium-mediated transformation system has been developed for introducing
foreign genes into the three most important potato cultivars in New Zealand ('Ilam …

Transformation of Acinetobacter sp. Strain BD413(pFG4ΔnptII) with Transgenic Plant DNA in Soil Microcosms and Effects of Kanamycin on Selection of …

KM Nielsen, JD van Elsas, K Smalla - Applied and Environmental …, 2000 - Am Soc Microbiol
Here we show that horizontal transfer of DNA, extracted from transgenic sugar beets, to
bacteria, based on homologous recombination, can occur in soil. Restoration of a 317-bp …

Polymerase chain reaction analysis of transgenic plants contaminated byAgrobacterium

V Nain, R Jaiswal, M Dalal, B Ramesh… - Plant molecular biology …, 2005 - Springer
Agrobacterium-mediated genetic transformation is a method of choice for the development
of transgenic plants. The presence of latent Agrobacterium that multiplies in the plant tissue …