Non-invasive measurement of glucose and pyruvate uptake by individual human oocytes and preimplantation embryos

K Hardy, MAK Hooper, AH Handyside… - Human …, 1989 - academic.oup.com
Pyruvate and glucose uptake by 73 indivudial human oocytes and preimplantation embryos
was measured non-invasively, using an ultramicrofluorescence assay to analyse changes in …

Uptake of pyruvate by early human embryos determined by a non-invasive technique

HJ Leese, MAK Hooper, RG Edwards… - Human …, 1986 - academic.oup.com
Normal levels of pyruvate in freshly collected follicular fluid were found to be 0.45 mM, a
value˜ 4× that of serum. The utilization of pyruvate by human oocytes and early embryos has …

Non-invasive measurement of pyruvate and glucose uptake and lactate production by sigle human preimplantation embryos

AL Gott, K Hardy, RML Winston… - Human reproduction, 1990 - academic.oup.com
The consumption of pyruvate and glucose and the production of lactate by 40 single human
preimplantation embryos has been measured using a non-invasive technique. Twelve of the …

Pyruvate and glucose uptake by mouse ova and preimplantation embryos

HJ Leese, AM Barton - Reproduction, 1984 - rep.bioscientifica.com
A method has been devised to measure the uptake of nutrients by small numbers of mouse
ova and preimplantation embryos. The 1–5 embryos were incubated as a group at 37° C in …

Fertilization and early embryology: Comparison of pyruvate uptake by embryos derived from conception and non-conception natural cycles

K Turner, KL Martin, BJ Woodward… - Human …, 1994 - academic.oup.com
The uptake of pyruvate by human embryos derived from natural cycles in the first 24 h
following fertilization was examined. Since only one egg was obtained and therefore only …

Non-invasive measurement of nutrient uptake by single cultured pre-implantation mouse embryos

DK Gardner, HJ Leese - Human reproduction, 1986 - academic.oup.com
The uptake of pyruvate and glucose by single, cultured mouse embryos has been measured
non-invasively throughout the pre-implantation period. The embryos, derived from CBA× …

Effects of pyruvate and glucose on the development of human preimplantation embryos in vitro

J Conaghan, AH Handyside, RML Winston… - …, 1993 - rep.bioscientifica.com
Although human embryos will develop in vitro for six days or more, little is known about the
effects of the primary nutrients, pyruvate and glucose, on development. Because the nutrient …

Assessment of embryo viability prior to transfer by the noninvasive measurement of glucose uptake

DK Gardner, HJ Leese - Journal of Experimental Zoology, 1987 - Wiley Online Library
An ultramicrofluorometric technique was used to measure the uptake of glucose by single
mouse blastocysts prior to transferring them into the uterus of pseudopregnant foster …

Uptake and metabolism of pyruvate and glucose by individual sheep preattachment embryos developed in vivo

DK Gardner, M Lane, P Batt - Molecular reproduction and …, 1993 - Wiley Online Library
The uptake of pyruvate and glucose by individual sheep oocytes and preattachment sheep
embryos at each state of development up to the hatching blastocyst was determined using a …

Early human embryo metabolism

HJ Leese, J Conaghan, KL Martin, K Hardy - Bioessays, 1993 - Wiley Online Library
Non‐invasive microanalytical methods have been devised to study the energy metabolism
of single human preimplantation embryos. Psyruvate, which is added routinely to all media …