Ribosomal RNA (rRNA) transcription is regulated primarily at the level of initiation from rRNA
promoters. The unusual kinetic properties of these promoters result in their specific …

Escherichia coli DksA, GreA, and GreB have similar structures and bind to the same location
on RNA polymerase (RNAP), the secondary channel. We show that GreB can fulfil some …

The Escherichia coli DksA protein inserts into the RNA polymerase (RNAP) secondary
channel, modifying the transcription initiation complex so that promoters with specific kinetic …

We show here that the promoters for many of the Escherichia coli ribosomal protein operons
are regulated directly by two transcription factors, the small RNA polymerase-binding protein …

▪ Abstract Ribosomal RNA transcription is the rate-limiting step in ribosome synthesis in
bacteria and has been investigated intensely for over half a century. Multiple mechanisms …

Amino acid starvation in Escherichia coli results in a spectrum of changes in gene
expression, including inhibition of rRNA and tRNA promoters and activation of certain …

DksA and ppGpp are the central players in the stringent response and mediate a complete
reprogramming of the transcriptome. A major component of the response is a reduction in …

Escherichia coli DksA is a transcription factor that binds to RNA polymerase (RNAP) without
binding to DNA, destabilizing RNAP–promoter interactions, sensitizing RNAP to the global …

Previous studies have come to conflicting conclusions about the requirement for the ω
subunit of RNA polymerase in bacterial transcription regulation. We demonstrate here that …

Bacterial transcription is regulated by the alarmone ppGpp, which binds near the catalytic
site of RNA polymerase (RNAP) and modulates its activity. We show that the DksA protein is …