Light-dependent generation of reactive oxygen species in cell culture media

A Grzelak, B Rychlik, G Bartosz - Free Radical Biology and Medicine, 2001 - Elsevier
Cell culture media (RPMI 1640, Dulbecco's Minimal Essential Medium and yeast extract-
peptone-glucose medium) were found to oxidize dichlorodihydrofluorescein diacetate and …

Reactive oxygen species: The good and the bad

R Patel, L Rinker, J Peng… - Reactive oxygen species …, 2018 - books.google.com
This chapter summarizes recent research on the biology of reactive oxygen species (ROS).
The chapter is focused on the bimodal actions of ROS, which can be summarized as both …

Effects of reactive oxygen species on proliferation of Chinese hamster lung fibroblast (V79) cells

BY Kim, MJ Han, AS Chung - Free Radical Biology and Medicine, 2001 - Elsevier
Reactive oxygen species (ROS) have emerged as important signaling molecules in the
regulation of various cellular processes. In our study, we investigated the effect of a wide …

Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader

H Wang, JA Joseph - Free radical biology and medicine, 1999 - Elsevier
Oxidative stress (OS) has been implicated in various degenerative diseases in aging. In an
attempt to quantify OS in a cell model, we examined OS induced by incubating for 30 min …

Photo-oxidation of cells generates long-lived intracellular protein peroxides

A Wright, CL Hawkins, MJ Davies - Free Radical Biology and Medicine, 2003 - Elsevier
Singlet oxygen is generated by several cellular, enzymatic, and chemical reactions as well
as by exposure to UV or visible light in the presence of a sensitizer. Consequently, this …

Irradiation of cells with ultraviolet-A (320-400 nm) in the presence of cell culture medium elicits biological effects due to extracellular generation of hydrogen peroxide

A Mahns, I Melchheier, CV Suschek, H Sies… - Free radical …, 2003 - Taylor & Francis
Biological effects of ultraviolet A (UVA) irradiation have been ascribed to the photochemical
generation of singlet oxygen. Not all effects described in the literature, however, are …

Altered cytotoxicity of ROS-inducing compounds by sodium pyruvate in cell culture medium depends on the location of ROS generation

JL Kelts, JJ Cali, SJ Duellman, J Shultz - Springerplus, 2015 - Springer
Induction of oxidative stress by drugs and other xenobiotics is an important mechanism of
cytotoxicity. However, in vitro studies on the relationship between oxidative stress and …

Reactive oxygen species: current knowledge and applications in cancer research and therapeutic

ATY Lau, Y Wang, JF Chiu - Journal of cellular biochemistry, 2008 - Wiley Online Library
Reactive oxygen species (ROS) are natural products inevitably generated along cellular
metabolism. Due to their highly reactive nature, which can damage DNA, proteins and lipids …

Methods for the detection of reactive oxygen species

Y Zhang, M Dai, Z Yuan - Analytical Methods, 2018 - pubs.rsc.org
There are many pieces of evidence suggesting the mediating role of reactive oxygen
species (ROS) in cell life, stress and death, and it is known that ROS at different …

Ultraviolet light induced generation of reactive oxygen species

TL De Jager, AE Cockrell, SS Du Plessis - Ultraviolet light in human health …, 2017 - Springer
As ultraviolet (UV) radiation is naturally and ubiquitously emitted by the sun, almost
everyone is exposed to it on a daily basis, and it is necessary for normal physiological …