RNA-mediated Allosteric Activation in CRISPR-Cas13a
S Sinha, AM Molina Vargas, PR Arantes, A Patel… - bioRxiv, 2023 - biorxiv.org
Cas13a is a recent addition to the CRISPR-Cas toolkit that exclusively targets RNA, which
makes it a promising tool for RNA detection. The protein uses a CRISPR RNA (crRNA) to …
makes it a promising tool for RNA detection. The protein uses a CRISPR RNA (crRNA) to …
Unveiling the RNA-mediated allosteric activation discloses functional hotspots in CRISPR-Cas13a
S Sinha, AM Molina Vargas, PR Arantes… - Nucleic Acids …, 2024 - academic.oup.com
Cas13a is a recent addition to the CRISPR-Cas toolkit that exclusively targets RNA, which
makes it a promising tool for RNA detection. It utilizes a CRISPR RNA (crRNA) to target RNA …
makes it a promising tool for RNA detection. It utilizes a CRISPR RNA (crRNA) to target RNA …
Defining the allosteric role of RNA in the CRISPR-associated Cas13a protein activation
S Sinha, AMM Vergas, PR Arantes, AC Patel… - Biophysical …, 2023 - cell.com
Cas13a is one of the latest additions in the CRISPR-Cas library that exclusively targets RNA,
a remarkable property being leveraged for the development of tools for RNA detection …
a remarkable property being leveraged for the development of tools for RNA detection …
CRISPR-RNA binding drives structural ordering that primes Cas7-11 for target cleavage
ABSTRACT Type III-E CRISPR-Cas effectors, referred to as Cas7-11 of gRAMPs, are single
proteins that cleave target RNAs without nonspecific collateral cleavage, opening new …
proteins that cleave target RNAs without nonspecific collateral cleavage, opening new …
[HTML][HTML] RNA binding and HEPN-nuclease activation are decoupled in CRISPR-Cas13a
CRISPR-Cas13a enzymes are RNA-guided, RNA-activated RNases. Their properties have
been exploited as powerful tools for RNA detection, RNA imaging, and RNA regulation …
been exploited as powerful tools for RNA detection, RNA imaging, and RNA regulation …
Atomistic understanding of the RNA-mediated allosteric crosstalk in Cas13a
Cas13a is one of the latest additions in the CRISPR-Cas library that exclusively targets RNA,
a remarkable property being leveraged for the development of tools for RNA detection …
a remarkable property being leveraged for the development of tools for RNA detection …
[HTML][HTML] Structural basis for the activation of a compact CRISPR-Cas13 nuclease
The CRISPR-Cas13 ribonucleases have been widely applied for RNA knockdown and
transcriptional modulation owing to their high programmability and specificity. However, the …
transcriptional modulation owing to their high programmability and specificity. However, the …
[HTML][HTML] RNA structure modulates Cas13 activity and enables mismatch detection
O Kimchi, BB Larsen, ORS Dunkley, AJW Te Velthuis… - bioRxiv, 2023 - ncbi.nlm.nih.gov
The RNA-targeting CRISPR nuclease Cas13 has emerged as a powerful tool for
applications ranging from nucleic acid detection to transcriptome engineering and RNA …
applications ranging from nucleic acid detection to transcriptome engineering and RNA …
CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells
CRISPR/Cas13 effectors have garnered increasing attention as easily customizable tools for
detecting and depleting RNAs of interest. Near perfect complementarity between a target …
detecting and depleting RNAs of interest. Near perfect complementarity between a target …
Engineering improved Cas13 effectors for targeted post-transcriptional regulation of gene expression
Cas13 is a family of unique RNA-targeting CRISPR-Cas effectors, making it an appealing
tool for probing and perturbing RNA function. However only a few Cas13 homologs have …
tool for probing and perturbing RNA function. However only a few Cas13 homologs have …