Characterization of spermatozoon viability is a common test in treating infertility. Recently, it
has been shown that label-free, phase-sensitive imaging can provide a valuable alternative …

Objective To compare label-free interferometric phase microscopy (IPM) to label-free and
label-based bright-field microscopy (BFM) in evaluating sperm cell morphology. This …

We developed a new method to identify the separate cellular compartments in the optical
path delay (OPD) maps of un‐labeled spermatozoa. This was conducted by comparing OPD …

Most living cells are optically transparent which makes it difficult to visualize them under
bright field microscopy. Use of contrast agents or markers and staining procedures are often …

Acridine orange (AO) staining is used to diagnose DNA fragmentation status in sperm cells.
Interferometric phase microscopy (IPM) is an optical imaging method based on digital …

Currently, the delicate process of selecting sperm cells to be used for in vitro fertilization
(IVF) is still based on the subjective, qualitative analysis of experienced clinicians using non …

We present results from non-interferometric quantitative phase imaging (QPI) for thickness
estimation and morphological analysis of human spermatozoa. QPI along with algorithms for …

The ability to evaluate sperm at the microscopic level, at high-throughput, would be useful
for assisted reproductive technologies (ARTs), as it can allow specific selection of sperm …

We suggest a new multimodal imaging technique for quantitatively measuring the integral
(thickness-average) refractive index of the nuclei of live biological cells in suspension. For …

The selection of sperm cells possessing normal morphology and motility is crucial for many
assisted reproductive technologies (ART), especially for intracytoplasmic sperm injection …