… cells in which mutants defective in several actin-binding proteins have been … a mutant
deficient in the 120-kD gelation factor, one of the most abundant F-actin cross-linking proteins of D. …

… thermotaxis assays with mutants lacking each of the five actin-binding proteins under study.
… actin cross-linking protein ABP-280 and three Dictyostelium actin-binding proteins used in …

… gelation factor-specific peaks of activity were absent in the double mutant, and no additional
F-actin cross-linking … Actin-binding proteins like a-actinin, the 120 kd gelation factor, severin, …

… lacking cross-linking proteins. The additive and synthetic phenotypes of these mutations
indicate that actin cross-linking proteins … of the F-actin cross-linking gelation factor (ABP-120). …

… Using the cytoskeleton mutants mentioned above, we looked for … In strains lacking the 120
kDa gelation factor, we found reduced … A Dictyostelium mutant deficient in severin, an F-actin …

… 120-kD gelation factor and a-actinin are among the most abundant F-actin cross-linking
proteins in Dictyostelium … of mutants with multiple defects in actin-binding proteins could clarify …

… , a calcium-regulated actin crosslinking protein, were created … cells lacking the 120-kD protein
derived from Dictyostelium AX3 … protein that gels F-actin in the cell cortex of Dictyostelium …

… gene in strain HGll30, we generated cells that lacked two F-actin cross-linking proteins. The
double … A Dictyostelium mutant lacking an F-actin cross-linking protein, the 120-kd gelation …

… We generated Dictyostelium double mutants lacking the two F-actin crosslinking proteins
α-actinin and gelation factor by inactivating the corresponding genes via homologous …

… Cross-linking proteins that form isotropic F-actin gels such as filamin, spectrin, and the
Dictyostelium 120-kD protein … This distinction is one major factor that must be taken into account. …