… , suggesting the existence in vivo of a large number of cullin-RING ubiquitin ligases. … the
RING finger domains allosterically activate E2. We suggest that RING-E2, rather than cullin-RING…

… (Citation13) showed that both Ubc5 and Cdc34 were … required for the assembly of the
ROC1-CUL1 core Ub ligase, we … between ROC1 and the cullin box of CUL1 is required for the …

… the ubiquitin-conjugating enzyme (E2) to the ubiquitin ligase … through Skp1 to assemble
various SCF–Roc1 E3 ligases that each … –Cul3–Roc1 complex, ubiquitin or E2–Ubc5 abolished …

… In vitro, E2F1 can be ubiquitinated by E2/Ubc5 but not by E2/… CUL5, constitute active ubiquitin
ligases with ROC1 and ROC2, … ROCs and cullins and ROC-cullin complex assembly was …

… It recruits ubiquitin-conjugating enzymes (E2s) and activates … the RING subunit recruits
the ubiquitin-conjugating enzyme (… proteins assemble with cullins to form ubiquitin ligases, a …

… be commonly utilized for the assembly of other cullin ligases. … activity that is dependent on
both E1 and E2-Ubc5 (Fig. 7A, … We suggest that the assembly of active CUL1 ligase is not …

… , each Ubl is activated by a specific E1 enzyme. Several groups … is the RBX1/Roc1 protein,
which together with the cullin, is a … CSN could assemble and presumably bind to cullin, the …

… -enzyme cascade involving the ubiquitin-activating enzyme (E1), a ubiquitin-conjugating
enzyme (E2), and a ubiquitin-… catalytic core, the formation of various active SCF complexes must …

… cycles of assembly and disassembly of different substrate receptor subunits from CDLs. …
C-terminus with Roc1 or 2 and a specific ubiquitin conjugating enzyme (Ubc3, Ubc4 or Ubc5). …

… Cullins assemble a potentially large number of ubiquitin … CDT1 for ubiquitination by a
CUL4A-dependent ubiquitin ligase, … ROC proteins activate an E2 ubiquitin-conjugating enzyme to …