Deconstructing complexity: serial block-face electron microscopic analysis of the hippocampal mossy fiber synapse

SA Wilke, JK Antonios, EA Bushong… - Journal of …, 2013 - Soc Neuroscience
… most complex synaptic structures in … electron microscopy techniques to quickly and accurately
reconstruct large volumes of neuropil. Here we use serial block-face electron microscopy (…

Examining hippocampal mossy fiber synapses by 3D electron microscopy in wildtype and Kirrel3 knockout mice

EA Martin, D Woodruff, RL Rawson, ME Williams - eneuro, 2017 - eneuro.org
serial block-face scanning electron microscopy (SBEM) with 3D reconstruction to define the
precise connectivity of MF filopodia and elucidate synaptic … the MF presynaptic complex, DG …

Structure, biophysics, and circuit function of a “giant” cortical presynaptic terminal

D Vandael, P Jonas - Science, 2024 - science.org
… Thus, the function of the hippocampal mossy fiber synapse may be more complex. … Electron
microscopy analysis revealed that large mossy fiber terminals form multiple active zones, …

[HTML][HTML] Three-dimensional structure of dendritic spines revealed by volume electron microscopy techniques

LK Parajuli, M Koike - Frontiers in Neuroanatomy, 2021 - frontiersin.org
… scanning electron microscopy; SBF-SEM, serial block-face scanning … of mossy fiber inputs
onto CA3 thorny excrescence spines, where multiple synaptic inputs (as many as 37 synaptic

[HTML][HTML] Specific disruption of hippocampal mossy fiber synapses in a mouse model of familial Alzheimer's disease

SA Wilke, T Raam, JK Antonios, EA Bushong, EH Koo… - PloS one, 2014 - journals.plos.org
… Since the mossy fiber (MF) synapse between dentate gyrus (… serial block-face electron
microscopy (SBEM) to analyze MF … MF synapse, we also reconstructed these complex structures …

Correlative light and volume electron microscopy to study brain development

S Hayashi, N Ohno, G Knott, Z Molnár - Microscopy, 2023 - academic.oup.com
… as well as in studying complex synaptic connections in thalamic … Serial block-face scanning
electron microscopy (SBF-SEM) [… multiple synapses with the connecting mossy fibre boutons […

[HTML][HTML] Reconstruction of neural circuits using serial block-face scanning electron microscopy

GH Kim, SH Lee, KJ Lee - Applied Microscopy, 2016 - appmicro.org
… reconstruction procedure of synaptic network in the rat hippocampal CA1 area and … the
serial section transmission electron microscopy, serial block-face scanning electron microscopy

Serial block face scanning electron microscopy—the future of cell ultrastructure imaging

L Hughes, C Hawes, S Monteith, S Vaughan - Protoplasma, 2014 - Springer
… Unravelling the connectivity of a network of neurons and the synaptic connections has … in
the study of the hippocampal mossy fiber terminal; one of the most complex synaptic structures …

Age-related changes in synaptic plasticity associated with mossy fiber terminal integration during adult neurogenesis

KD Murray, XB Liu, AN King, JD Luu, HJ Cheng - Eneuro, 2020 - eneuro.org
electron microscopy (EM), we describe the maturation of … We therefore focused these analyses
on the large complex … In addition, a recent serial block face EM reconstruction analysis in …

[PDF][PDF] An input-specific orphan receptor GPR158-HSPG interaction organizes hippocampal mossy fiber-CA3 synapses

G Condomitti, KD Wierda, A Schroeder, SE Rubio… - Neuron, 2018 - cell.com
… Neuron-derived, presynaptic GPC4 forms a trans-synaptic complex with the postsynaptic …
serial block-face scanning electron microscopy (SBF-SEM) (Figures 5L and S6E). Analysis of …