… coli is a suitable expression host for large-scale production of … , the chicken IFN-β mature
protein gene linked it with 6-His tag codons at its C-terminus was cloned and expressed it in E. …

… GBP-inducing activity, we chose a cDNA expression cloning … positive clone revealed that
it encodes chicken interferon-y (… been purified, we do not know whether the specific activity of …

… the innate and adaptive immune system. Furthermore, they … activity to inhibit the cytopathic
effect caused by hand, foot, … at positive control, the antivirus activity of the recombinant chIFN-α …

… antivirus bioactivity as compared to ChIFN-β and IFN-γ, especially with respect to early immune
… All the viruses were plaque-purified three times on primary chicken embryo fibroblasts, …

… in Escherichia coli, was purified by Ni chromatography, and … this problem, including immaturity
of their immune system"' and stress … One unit per mil¬ liliter of IFN activity is defined as the …

… the signal peptide, was purified to homogeneity from the … as reciprocals of the dilutions
giving 50 % virus protection. … antiserum and recovered the immune complexes with protein-A-…

… replication and stimulation of the immune response (Citation[1]… The anti-virus titer from five
lots of rSH-ChIFN-α and rWJ-ChIFN… The purified rSH-ChIFN-α has an anti-VSV activity of more …

… It was also the first cytokine to be purified to homogeneity, cloned, … In chicken, there appears
to be only a single copy of IFN-λ [4]… of its antiviral activity in vivo via stimulation of the immune …

… may increase the complexity of the immune response and cause … since extraction times
differed for sample A and B from … 3 U/mLChIFN-γ protein anti-virus titer (ie the amount of CPE …

… This indicates that the immune systems of chickens, ducks, … effect of GoIRF7 on the expression
of innate immune genes, we … are pivotal for inducing IFN-β promoter activity and that the …