… Our σ 28 prediction algorithm can be applied to other bacteria… Escherichia coli genome, we
have predicted and functionally identified five previously unknown σ 28 -regulated genes in E…

… products connected with various critical biological functions … to nitrogen levels in Escherichia
coli and Salmonella (1, 4). It … and to validate the σ 54 promoter for these genes, primers …

… in Escherichia coli σ 70 . Conversely, residue Q236 of σ 28 Ct directly binds the chlamydial
… As validation, we also examined potential core-binding differences among these proteins …

… In the absence of robust gene knockdown techniques for Chlamydia [45], we took the
overexpression approach to probe the organismal function of GrgA. In our initial attempt to …

… to validate the … predict the expression of these genes was likewise upregulated. Our
qRT-PCR analysis also confirmed expression increases of four non-operon genes functionally …

… the Escherichia coli housekeeping σ 70 ), two alternative sigma factors have been identified
in the genome but their functions are … Several predicted and validated promoter motifs have …

… chlamydial sigma factors (σ 66 , σ 28 , and σ 54 ) that recognize promoter sequences of genes,
chlamydial … Here, we investigate the function of GrgA, a Chlamydia -specific transcription …

… , promoter sequence analysis, and transcription report assays in Escherichia coli (11). T,
targets defined by … These functions validate GrgA’s role as a master transcriptional regulator in …

… Computational, genetic, and biochemical analyses demonstrated that TDE2683 has a …
Escherichia coli, binds to σ 28 -dependent promoters, and can functionally replace the σ 28 of E. …

… pneumoniae J138 16 and focused on analysis of the mid to late stage of chlamydial gene …
similar sequences to the −35 consensus of Escherichia coli σ 28 promoter at approximately 60 …