Live-imaging of plant development: latest approaches

PG Sappl, MG Heisler - Current opinion in plant biology, 2013 - Elsevier
… illustrate the latest advances in the application of live-imaging to dissect plant development.
New approaches to live-imaging at the subcellular scale have provided important insights …

Live imaging of root–bacteria interactions in a microfluidics setup

H Massalha, E Korenblum, S Malitsky… - Proceedings of the …, 2017 - National Acad Sciences
… Here, we describe a microfluidics-based approach enabling direct imaging of root–bacteria …
Although several platforms for live imaging of plant roots have been reported, they were …

High‐resolution live imaging of plant growth in near physiological bright conditions using light sheet fluorescence microscopy

A Maizel, D von Wangenheim, F Federici… - The Plant …, 2011 - Wiley Online Library
… We demonstrate the applicability of our approach at varying spatial and temporal scales by
… microscopy, our approach presents several advantages for studying plant growth. The main …

Unravelling developmental dynamics: transient intervention and live imaging in plants

GV Reddy, SP Gordon, EM Meyerowitz - Nature Reviews Molecular Cell …, 2007 - nature.com
… using transient perturbation and live-imaging methods. … live imaging and transient perturbation
technologies and tools that are being used to study plant physiology and developmental

Live-Imaging and Image Processing of Shoot Apical Meristems of Arabidopsis thaliana

GV Reddy, A Roy-Chowdhury - Plant Systems Biology, 2009 - Springer
… This effort has involved the development of new live-imaging methods, new fluorescent
reporter … the live-imaging methods and image processing methods that have been developed to …

Live-imaging of the Arabidopsis inflorescence meristem

MG Heisler, C Ohno - Flower Development: Methods and Protocols, 2014 - Springer
… The SAM is therefore of prime interest for understanding plant growth and development.
In this chapter we outline methods for using confocal microscopy to image the Arabidopsis …

The mechanics behind plant development

O Hamant, J Traas - New Phytologist, 2010 - Wiley Online Library
approaches have been used to understand how these cellular processes are integrated
in the growing tissues. These include quantitative live imaging to measure growth rate and …

Multiscale imaging of plant development by light-sheet fluorescence microscopy

M Ovečka, D von Wangenheim, P Tomančák… - Nature Plants, 2018 - nature.com
methods in developmental studies of established and emerging plant model species, together
with up-to-date methods … LSFM is ideally suited for live imaging of Arabidopsis, and the …

Live biospeckle laser imaging of root tissues

RA Braga, L Dupuy, M Pasqual… - European biophysics …, 2009 - Springer
Live imaging is now a central component for the study of plant developmental processes. …
Unfortunately, live imaging approaches are generally time-consuming, fastidious and highly …

Superresolution live imaging of plant cells using structured illumination microscopy

G Komis, M Mistrik, O Šamajová, M Ovečka, J Bartek… - Nature protocols, 2015 - nature.com
… (SR) approaches have been … in plant cells still remains under-developed. Here we describe
a validated method for time-lapse SIM that focuses on cortical microtubules of different plant