[HTML][HTML] Metabolic fingerprinting of bacteria by fluorescence lifetime imaging microscopy

A Bhattacharjee, R Datta, E Gratton, AI Hochbaum - Scientific reports, 2017 - nature.com
… The identification of bacterial metabolic states and transitions between them in … fluorescence
lifetime imaging microscopy (FLIM) to create a metabolic fingerprint of individual bacteria

[PDF][PDF] FLIM Phasor Fingerprint of Bacterial Metabolic State

R Datta, A Bhattacharjee, A Hochbaum, E Gratton - Biophysical Journal, 2017 - cell.com
… rial metabolism could shed light on their physiological status. In this work we employ two-photon
fluorescence lifetime imaging microscopy (FLIM) of live, clinically relevant bacteria. …

[HTML][HTML] Fluorescence Lifetime Imaging Unravels C. trachomatis Metabolism and Its Crosstalk with the Host Cell

M Szaszák, P Steven, K Shima… - PLoS …, 2011 - journals.plos.org
metabolism in intracellular bacterial infections that exceeds sole competition for nutrients. In
conclusion, fluorescence lifetime imaging … with host cell metabolism, metabolic profiling of …

[图书][B] Label-free fluorescence lifetime imaging microscopy (FLIM) to study metabolism and oxidative stress in biological systems

R Datta - 2016 - search.proquest.com
fluorescence lifetime imaging microscopy (FLIM) of intrinsic fluorophores for label-free metabolic
and oxidative stress imaging … FLIM-phasor fingerprint of clinically important bacteria. We …

Fluorescence lifetime imaging microscopy (FLIM): a non-traditional approach to study host-microbial symbioses

P Deore, I Wanigasuriya, SJTM Ching… - Microbiology …, 2022 - CSIRO Publishing
… ) coupled with fluorescence lifetime imaging microscopy (FLIM… in situ location and identity
of bacteria in Symbiodiniaceae. … endogenous chemicals (eg metabolic co-enzymes, structural …

[HTML][HTML] Raman imaging and fluorescence lifetime imaging microscopy for diagnosis of cancer state and metabolic monitoring

L Becker, N Janssen, SL Layland, TE Muerdter… - Cancers, 2021 - mdpi.com
… -active [8] and thus provide molecular fingerprints that are highly sensitive and can reflect a
… modalities of these techniques for cancer imaging, metabolic monitoring, and diagnosis. In …

[HTML][HTML] Real-time, label-free detection and identification of bacteria through non-invasive optical imaging

F Qazi, R Verma, CE Redmond, A Khalid… - Microbes and …, 2024 - Elsevier
… spectra and fluorescence lifetimes can be used as a fingerprint for … fluorescence lifetime to
distinguish bacteria from each other. … We hypothesised that the metabolic differences between …

[HTML][HTML] Microglia activation visualization via fluorescence lifetime imaging microscopy of intrinsically fluorescent metabolic cofactors

MAK Sagar, JN Ouellette, KP Cheng… - …, 2020 - spiedigitallibrary.org
… free signatures for different cell types ranging from bacteria to stem cells. A strategy reported
… that mean NADH lifetime is well-suited for identifying a microglial fingerprint upon activation. …

A rapid analysis platform for investigating the cellular locations of bacteria using two-photon fluorescence lifetime imaging microscopy

N Sapermsap, DDU Li, R Al-Hemedawi… - … in Fluorescence, 2020 - iopscience.iop.org
… , a red-fluorescent by-product of bacterial haem production, and … Most interestingly, two-photon
FLIM imaging of the metabolic … been used for bacterial metabolic fingerprinting in diverse …

Label-free multiphoton imaging of microbes in root, mineral, and soil matrices with time-gated coherent Raman and fluorescence lifetime imaging

J Lee, R Hestrin, EE Nuccio, KD Morrison… - … science & technology, 2022 - ACS Publications
… for biomedical imaging and studies of cellular metabolism due … of imaging fluorescently
labeled bacteria, we imaged DAPI-… In future work, CARS bands from the so-called “fingerprint” …