… of selective acinar cell destruction was achieved. In contrast with conventional … enzyme-free
method of islet isolation relying on in situ cryopreservation of islets with simultaneous freeze …

… allows the banking of a large number of cells and tissues that … cryopreservation to maximize
post-cryo viability for each cell … freeze-thaw rates carries with it the potential to damage cells …

… examined islet isolation after 30 min WIT in situ without (G5, n … removal and freezing (0.5
ml), and islet resuspension in the … Figure 5C shows an intact islet surrounded by acinar tissue …

… , storage at low temperature, and culture (47,51). … a progressive loss of acinar tissue
surrounding the islets, which … well demarcated in situ but fragile, resulting in substantial islet losses …

… , however, involves the dissociation of pancreatic tissue by a … on acinar, duct, and islet cells
in the normal pancreas of the … in situ and in isolated islets. Bars: A,B = 18 μm; C,D = 22 μm. …

… tissue (34). Harmful enzymes that are released from dying acinar cells during culture may be
deleterious to islet cell … (due to the low temperature), even if they are released by the dying …

… Because islet isolation requires manipulation of human tissue… therapeutic option for diabetes,
islet transplantation, as a cell … of nonenzyme-based procedures for islet isolation 146,147 is …

… pancreas is crucial to the subsequent ability to isolate viable … approach is to perfuse the
pancreas via the pancreatic duct … , the amount of undigested tissue remaining after dissociation …

… and selective destruction of acinar tissue,” Transplantation … , “Nonenzymatic cryogenic
isolation of therapeutic cells: novel approach for enzyme-free isolation of pancreatic islets using in …

… Tissue dissociation enzymes for islet isolation have been extensively … Tissue samples were
taken from the body of the pancreas immediately after collagenase infusion and snap frozen …