Positive-and negative-control pathways by blood components for intermedilysin production in Streptococcus intermedius
T Tomoyasu, T Yamasaki, S Chiba, S Kusaka… - Infection and …, 2017 - Am Soc Microbiol
… Streptococcus intermedius is an opportunistic bacterial pathogen secreting a human-specific
cytolysin called intermedilysin … intermedius PC574 was cultured in fetal bovine serum (FBS) …
cytolysin called intermedilysin … intermedius PC574 was cultured in fetal bovine serum (FBS) …
[HTML][HTML] Identification of the high affinity binding site in the Streptococcus intermedius toxin intermedilysin for its membrane receptor, the human complement regulator …
… Intermedilysin (ILY) is a member of the CDC family produced by Streptococcus intermedius.
ILY is … profile is binding of Δ6eGFPsILY to sheep erythrocytes, included as a negative control. …
ILY is … profile is binding of Δ6eGFPsILY to sheep erythrocytes, included as a negative control. …
Characterization of Streptococcus milleri Group Isolates from Expectorated Sputum of Adult Patients with Cystic Fibrosis
ME Grinwis, CD Sibley, MD Parkins… - Journal of clinical …, 2010 - Am Soc Microbiol
… intermedius strains produce a potent human-specific cytoxin, intermedilysin, which shows
little to no activity … Sterile Todd-Hewitt-yeast broth was used as a negative control. Luciferase …
little to no activity … Sterile Todd-Hewitt-yeast broth was used as a negative control. Luciferase …
Rapid screening method for detecting highly pathogenic Streptococcus intermedius strains carrying a mutation in the lacR gene
T Tomoyasu, M Matoba, A Takao… - FEMS microbiology …, 2018 - academic.oup.com
… secretes a human-specific cytolysin intermedilysin (ILY) as a major … Because of the correlation
between higher production of ILY … Because MsgA and NanA activities showed positive and …
between higher production of ILY … Because MsgA and NanA activities showed positive and …
[HTML][HTML] From normal flora to brain abscesses: a review of Streptococcus intermedius
… Streptococcus intermedius is a β-hemolytic Gram-positive coccus and member of the
Streptococcus anginosus … intermedilysin and its 3’-flanking region was shown to be specific for S. …
Streptococcus anginosus … intermedilysin and its 3’-flanking region was shown to be specific for S. …
Streptolysin S of Streptococcus anginosus exhibits broad-range hemolytic activity
D Asam, S Mauerer, B Spellerberg - Medical microbiology and …, 2015 - Springer
… anginosus group is the intermedilysin, produced by S. … As a negative control, the two
pAT28::sagB vectors were … In contrast to intermedilysin, our experiments demonstrated the …
pAT28::sagB vectors were … In contrast to intermedilysin, our experiments demonstrated the …
Mechanisms of host recognition and immune evasion of members of the Streptococcus anginosus/milleri group.
K Giraldi - 2015 - macsphere.mcmaster.ca
… It was also found that intermedilysin expression by S. … the fold change in absorbance
relative to the negative control. … intermedius strains, which are known to produce ILY, and the …
relative to the negative control. … intermedius strains, which are known to produce ILY, and the …
A simple method to differentiate three classes of cholesterol-dependent cytolysins
T Tomoyasu, A Matsumoto, A Takao, A Tabata… - Journal of …, 2023 - Elsevier
… Only intermedilysin from Streptococcus intermedius has … μL erythrocyte suspension for the
positive control of hemolysis (… -inactivated plasma for the negative control of hemolysis (0% …
positive control of hemolysis (… -inactivated plasma for the negative control of hemolysis (0% …
Functional and Phylogenetic Characterization of Vaginolysin, the Human-Specific Cytolysin from Gardnerella vaginalis
SE Gelber, JL Aguilar, KLT Lewis… - Journal of …, 2008 - Am Soc Microbiol
… related to intermedilysin from Streptococcus intermedius. … The negative control for lysis
consisted of PBS without toxin … and IL-8 pathways in human epithelial cells, suggest that VLY …
consisted of PBS without toxin … and IL-8 pathways in human epithelial cells, suggest that VLY …
[HTML][HTML] Regulation of the β-hemolysin gene cluster of Streptococcus anginosus by CcpA
R Bauer, S Mauerer, B Spellerberg - Scientific reports, 2018 - nature.com
… Positive control: ddH 2 O; negative control: assay buffer. The … of alternative sugar utilization
pathways in the presence of … anginosus pBSU409 strain served as negative control 44 …
pathways in the presence of … anginosus pBSU409 strain served as negative control 44 …