Stability of a thermophilic TIM-barrel enzyme: indole-3-glycerol phosphate synthase from the thermophilic archaeon Sulfolobus solfataricus

G ANDREOTTI, MV CUBELLIS, M DI PALO… - Biochemical …, 1997 - portlandpress.com
… The stability and activity of indole-3-glycerol phosphate synthase from Sulfolobus solfataricus
… In this paper we focus on three points: (1) the longterm stability of the protein to irreversible …

Indole-3-glycerol-phosphate synthase from Sulfolobus solfataricus as a model for studying thermostable TIM-barrel enzymes

G Andreotti, ML Tutino, G Sannia, G Marino… - … et Biophysica Acta (BBA …, 1994 - Elsevier
Indole-3-glycerol-phosphate synthase, a thermophilic and thermostable enzyme from the
archaeon Sulfolobus solfataricus… the thermal stability of IndGroP synthaseSs, the enzyme (0.5 …

[PDF][PDF] 2.0 Å structure of indole-3-glycerol phosphate synthase from the hyperthermophile Sulfolobus solfataricus: possible determinants of protein stability

M Hennig, B Darimont, R Sterner, K Kirschner… - Structure, 1995 - cell.com
… are of special interest in TIM-barrel enzymes. The loops ai-13i+l are … Indole-3glycerol
phosphate synthase (IGPS) is a relatively … hyperthermophile Sulfolobus solfataricus (sIGPS) and …

Folding mechanism of indole-3-glycerol phosphate synthase from Sulfolobus solfataricus: a test of the conservation of folding mechanisms hypothesis in (βα) 8 barrels

WR Forsyth, CR Matthews - Journal of molecular biology, 2002 - Elsevier
… Over 30 different enzymes from all seven functional classes … Sulfolobus solfataricus (sIGPS)
is another TIM barrel that has … kinetic stabilization often displayed by thermophilic proteins 55 …

Role of the N-Terminal Extension of the (βα)8-Barrel Enzyme Indole-3-glycerol Phosphate Synthase for Its Fold, Stability, and Catalytic Activity,

B Schneider, T Knöchel, B Darimont, M Hennig… - Biochemistry, 2005 - ACS Publications
… variants of IGPS from Sulfolobus solfataricus [sIGPS ( 9)] and … with the hypothesis that
TIM-barrel enzymes involved in the … thermophilic indole-3-glycerol phosphate synthase enzymes

The crystal structure of indole-3-glycerol phosphate synthase from the hyperthermophilic archaeonsulfolobus solfataricusin three different crystal forms: effects of ionic …

TR Knöchel, M Hennig, A Merz, B Darimont… - Journal of molecular …, 1996 - Elsevier
… chaeon Sulfolobus solfataricus is a monomeric enzyme with … Comparison of orthologous
proteins from hyperthermophilic … ion-pair networks in maintaining enzyme stability at extreme …

Relationship of Catalysis and Active Site Loop Dynamics in the (βα)8-Barrel Enzyme Indole-3-glycerol Phosphate Synthase

S Schlee, T Klein, M Schumacher, J Nazet, R Merkl… - Biochemistry, 2018 - ACS Publications
… The (βα) 8 or TIM barrel is the most common single-domain enzyme fold in … enzyme, namely
indole-3-glycerol phosphate synthase from the hyperthermophile Sulfolobus solfataricus (…

The catalytic mechanism of indole-3-glycerol phosphate synthase: crystal structures of complexes of the enzyme from Sulfolobus solfataricus with substrate analogue …

M Hennig, BD Darimont, JN Jansonius… - Journal of molecular …, 2002 - Elsevier
… complexes of the hyperthermostable enzyme from Sulfolobus solfataricus with the substrate
… 5-phosphate, a substrate analogue and the product indole-3-glycerol phosphate. The …

The hyperthermostable indoleglycerol phosphate synthase from Thermotoga maritima is destabilized by mutational disruption of two solvent-exposed salt bridges

A Merz, T Knöchel, JN Jansonius… - Journal of molecular …, 1999 - Elsevier
Stability of a thermophilic TIM-barrel enzyme: indole-3-glycerol phosphate synthase from
the thermophilic archaeon Sulfolobus solfataricus

mechanistic analysis of the tryptophan biosynthetic enzyme indole-3-glycerol phosphate synthase

MJ Zaccardi - 2014 - etda.libraries.psu.edu
… the IGPS enzyme from the thermophile Sulfolobus sulfataricus (… Enzymes containing the
(β/α)8-barrel (or TIM-barrel, named … is a thermophilic enzyme; therefore, its increased stability is …