The key role of water in the dioxygenase function of Escherichia coli flavohemoglobin
… Flavohemoglobin dioxygenase function as studied in the present work, relies on ligand (oxygen
and nitric oxide) migration to the active site (left panel), chemical reaction between NO …
and nitric oxide) migration to the active site (left panel), chemical reaction between NO …
Nitric oxide dioxygenase function and mechanism of flavohemoglobin, hemoglobin, myoglobin and their associated reductases
PR Gardner - Journal of inorganic biochemistry, 2005 - Elsevier
… of Escherichia coli to radical dot NO poisoning and aconitase inactivation [49], [50] led my lab
to the biochemical and genetic identification of a flavoHb … NO dioxygenase (NOD) function (…
to the biochemical and genetic identification of a flavoHb … NO dioxygenase (NOD) function (…
[HTML][HTML] Hemoglobin: a nitric-oxide dioxygenase
PR Gardner - Scientifica, 2012 - hindawi.com
… NOD function of E. coli flavoHb. The observations offered a distinct and common enzymatic
… analyses [394] suggest an important role for water molecules bridging the FAD and heme in …
… analyses [394] suggest an important role for water molecules bridging the FAD and heme in …
Nitric oxide dioxygenase: an enzymic function for flavohemoglobin
PR Gardner, AM Gardner, LA Martin… - Proceedings of the …, 1998 - National Acad Sciences
… Previously, we demonstrated an NO • sensitivity of the Escherichia coli and the … water
molecule that are thought to act as an electron conduit (26). Spectroscopic studies of the E…
molecule that are thought to act as an electron conduit (26). Spectroscopic studies of the E…
Steady-state and transient kinetics of Escherichia coli nitric-oxide dioxygenase (flavohemoglobin): the B10 tyrosine hydroxyl is essential for dioxygen binding and …
AM Gardner, LA Martin, PR Gardner - Journal of Biological Chemistry, 2000 - ASBMB
… Escherichia coli expresses an inducible flavohemoglobin possessing robust NO dioxygenase
… Dioxygen binds tightly to reduced flavohemoglobin, with association and dissociation rate …
… Dioxygen binds tightly to reduced flavohemoglobin, with association and dissociation rate …
Bacterial Hemoglobins and Flavohemoglobins for Alleviation of Nitrosative Stress in Escherichia coli
AD Frey, J Farrés, CJT Bollinger… - Applied and …, 2002 - Am Soc Microbiol
… to water (36). In particular, reduced flavoproteins have been implicated in this redox process
in Escherichia coli (… One is a route including a dioxygenase (11, 16), where the heme-bound …
in Escherichia coli (… One is a route including a dioxygenase (11, 16), where the heme-bound …
Flavohemoglobin detoxifies nitric oxide in aerobic, but not anaerobic, Escherichia coli: evidence for a novel inducible anaerobic nitric oxide-scavenging activity
AM Gardner, PR Gardner - Journal of Biological Chemistry, 2002 - ASBMB
… Nitric-oxide dioxygenase (NOD) and reductase (NOR) activities of flavohemoglobin (flavoHb…
of yeast extract, and 10 g of NaCl in 1 liter of water containing 66 mm K 2 HPO 4 and 33 mm …
of yeast extract, and 10 g of NaCl in 1 liter of water containing 66 mm K 2 HPO 4 and 33 mm …
Allostery in the nitric oxide dioxygenase mechanism of flavohemoglobin
AM Gardner, PR Gardner - Journal of Biological Chemistry, 2021 - ASBMB
… activate the NO dioxygenase function of Escherichia coli flavohemoglobin. Steady-state and
… E and F-helices and switches an electron transfer gate formed by LysF7, GlnE7, and water. …
… E and F-helices and switches an electron transfer gate formed by LysF7, GlnE7, and water. …
Nitric-oxide dioxygenase activity and function of flavohemoglobins: sensitivity to nitric oxide and carbon monoxide inhibition
PR Gardner, AM Gardner, LA Martin, Y Dou, T Li… - Journal of Biological …, 2000 - ASBMB
… defenses in Escherichia coli led to the isolation of an NO-inducible NAD(P)H, O 2 , and
FAD-dependent NOD 1 activity. This enzyme activity was identified with the flavoHb encoded by …
FAD-dependent NOD 1 activity. This enzyme activity was identified with the flavoHb encoded by …
Flavohemoglobin: structure and reactivity
A Bonamore, A Boffi - IUBMB life, 2008 - Wiley Online Library
… flavoHb from Escherichia coli (HMP) has been the object of a large number of investigations
to unveil its physiological role … of NO to NO 3 − (NO-dioxygenase activity) both in vivo and in …
to unveil its physiological role … of NO to NO 3 − (NO-dioxygenase activity) both in vivo and in …