The synergism of stem cell biology and biomaterial technology promises to have a profound
impact on stem-cell-based clinical applications for tissue regeneration. Biomaterials …

The widespread clinical implementation of gene therapy requires the ability to stably
integrate genetic information through gene transfer vectors in a safe, effective, and …

Over the past decade, the Sleeping Beauty (SB) transposon system has been developed as
the leading non-viral vector for gene therapy. This vector combines the advantages of …

Molecular medicine has entered a high-tech age that provides curative treatments of
complex genetic diseases through genetically engineered cellular medicinal products. Their …

Transposable elements can be viewed as natural DNA transfer vehicles that, similar to
integrating viruses, are capable of efficient genomic insertion. The mobility of class II …

Transposons derived from Sleeping Beauty (SB), piggyBac (PB), or Tol2 typically require
cotransfection of transposon DNA with a transposase either as an expression plasmid or …

Transposons have emerged as promising vectors for gene therapy that can potentially
overcome some of the limitations of commonly used viral vectors. Transposons stably …

The discovery of direct cell reprogramming and induced pluripotent stem (iPS) cell
technology opened up new avenues for the application of non-viral, transposon-based gene …

The domestic pig is an important large animal model for preclinical testing of novel cell
therapies. Recently, we produced pluripotency reporter pigs in which the Oct4 promoter …

There is significant interest in the use of mesenchymal stem cells (MSCs) as a potential
therapeutic modality in disease and disorder, particularly those with an inflammation-based …