RNA-based gene therapy requires therapeutic RNA to function inside target cells without
eliciting unwanted immune responses. RNA can be ferried into cells using non-viral drug …

CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …

Gene-editing technologies, which include the CRISPR–Cas nucleases 1, 2, 3 and CRISPR
base editors 4, 5, have the potential to permanently modify disease-causing genes in …

RNA-guided systems, which use complementarity between a guide RNA and target nucleic
acid sequences for recognition of genetic elements, have a central role in biological …

Climate change gives rise to numerous environmental stresses, including soil salinity.
Salinity/salt stress is the second biggest abiotic factor affecting agricultural productivity …

The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …

Multiple clinical trials of allogeneic T cell therapy use site-specific nucleases to disrupt T cell
receptor (TCR) and other genes,,,,–. In this study, using single-cell RNA sequencing, we …

The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an
ongoing global pandemic due in part to the challenge of identifying symptomatic …

IscB proteins are putative nucleases encoded in a distinct family of IS200/IS605 transposons
and are likely ancestors of the RNA-guided endonuclease Cas9, but the functions of IscB …