Precise plant genome editing using base editors and prime editors
The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
[HTML][HTML] Strategies to overcome the main challenges of the use of CRISPR/Cas9 as a replacement for cancer therapy
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats-associated protein
9) shows the opportunity to treat a diverse array of untreated various genetic and …
9) shows the opportunity to treat a diverse array of untreated various genetic and …
Enhancing grain-yield-related traits by CRISPR–Cas9 promoter editing of maize CLE genes
Several yield-related traits selected during crop domestication and improvement, are
associated with increases in meristem size, which is controlled by CLE peptide signals in the …
associated with increases in meristem size, which is controlled by CLE peptide signals in the …
Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9
X Ma, X Zhang, H Liu, Z Li - Nature Plants, 2020 - nature.com
Genome-editing technologies using CRISPR–Cas nucleases have revolutionized plant
science and hold enormous promise in crop improvement. Conventional transgene …
science and hold enormous promise in crop improvement. Conventional transgene …
Domestication of wild tomato is accelerated by genome editing
T Li, X Yang, Y Yu, X Si, X Zhai, H Zhang, W Dong… - Nature …, 2018 - nature.com
Crop improvement by inbreeding often results in fitness penalties and loss of genetic
diversity. We introduced desirable traits into four stress-tolerant wild-tomato accessions by …
diversity. We introduced desirable traits into four stress-tolerant wild-tomato accessions by …
[HTML][HTML] Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation
Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is a destructive citrus disease
worldwide. Generating disease-resistant cultivars is the most effective, environmentally …
worldwide. Generating disease-resistant cultivars is the most effective, environmentally …
A single gene underlies the dynamic evolution of poplar sex determination
Although hundreds of plant lineages have independently evolved dioecy (that is, separation
of the sexes), the underlying genetic basis remains largely elusive. Here we show that …
of the sexes), the underlying genetic basis remains largely elusive. Here we show that …
Computational tools and resources for CRISPR/Cas genome editing
The past decade has witnessed a rapid evolution in identifying more versatile clustered
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
Targeted, efficient sequence insertion and replacement in rice
Y Lu, Y Tian, R Shen, Q Yao, M Wang, M Chen… - Nature …, 2020 - nature.com
CRISPR–Cas9 methods have been applied to generate random insertions and deletions,
large deletions, targeted insertions or replacements of short sequences, and precise base …
large deletions, targeted insertions or replacements of short sequences, and precise base …
CRISPR/Cas systems in genome editing: methodologies and tools for sgRNA design, off‐target evaluation, and strategies to mitigate off‐target effects
H Manghwar, B Li, X Ding, A Hussain… - Advanced …, 2020 - Wiley Online Library
Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger
nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory …
nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory …