A microsomal butyrylesterase appearing in rat livers during development, regeneration, and carcinogenesis, and after phenobarbital treatment

A Kaneko, N Chisaka, K Enomoto… - Journal of the …, 1979 - academic.oup.com
A Kaneko, N Chisaka, K Enomoto, T Kaku, K Dempo, M Mori, T Onoé
Journal of the National Cancer Institute, 1979academic.oup.com
A microsomal butyrylesterase (LI) was purified from the livers of male W rats treated with
phenobarbital, and an antiserum against this purified LI was raised in a rabbit. By the
Ouchteriony double-diffusion test, a precipitin line was observed between the anti-LI
antiserum and each Triton X-100 extract of livers during development, regeneration after
partial hepatectomy, and carcinogenesis and of hyperplastic nodules and hepatomas, all of
which revealed LI in their esterase isoenzyme patterns. These precipitin lines exhibited …
Abstract
A microsomal butyrylesterase (L-I) was purified from the livers of male W rats treated with phenobarbital, and an antiserum against this purified L-I was raised in a rabbit. By the Ouchteriony double-diffusion test, a precipitin line was observed between the anti-L-I antiserum and each Triton X-100 extract of livers during development, regeneration after partial hepatectomy, and carcinogenesis and of hyperplastic nodules and hepatomas, all of which revealed L-I in their esterase isoenzyme patterns. These precipitin lines exhibited esterase activity. The fusion of the lines of these tissue extracts and that of the purified L-I indicated the presence of an antigen site common to their esterases. The extracts of adult and fetal livers and also of hepatomas resembling fetal liver in the esterase isoenzyme pattern did not produce a precipitin line with anti-L-I antiserum.
Oxford University Press
以上显示的是最相近的搜索结果。 查看全部搜索结果