A prominent role for the CBF cold response pathway in configuring the low-temperature metabolome of Arabidopsis

D Cook, S Fowler, O Fiehn… - Proceedings of the …, 2004 - National Acad Sciences
D Cook, S Fowler, O Fiehn, MF Thomashow
Proceedings of the National Academy of Sciences, 2004National Acad Sciences
The Arabidopsis CBF cold response pathway has a central role in cold acclimation, the
process whereby plants increase in freezing tolerance in response to low nonfreezing
temperatures. Here we examined the changes that occur in the Arabidopsis metabolome in
response to low temperature and assessed the role of the CBF cold response pathway in
bringing about these modifications. Of 434 metabolites monitored by GC-time-of-flight MS,
325 (75%) were found to increase in Arabidopsis Wassilewskija-2 (Ws-2) plants in response …
The Arabidopsis CBF cold response pathway has a central role in cold acclimation, the process whereby plants increase in freezing tolerance in response to low nonfreezing temperatures. Here we examined the changes that occur in the Arabidopsis metabolome in response to low temperature and assessed the role of the CBF cold response pathway in bringing about these modifications. Of 434 metabolites monitored by GC-time-of-flight MS, 325 (75%) were found to increase in Arabidopsis Wassilewskija-2 (Ws-2) plants in response to low temperature. Of these 325 metabolites, 256 (79%) also increased in nonacclimated Ws-2 plants in response to overexpression of C-repeat/dehydration responsive element-binding factor (CBF)3. Extensive cold-induced changes also occurred in the metabolome of Arabidopsis Cape Verde Islands-1 (Cvi-1) plants, which were found to be less freezing tolerant than Ws-2 plants. However, low-temperature-induced expression of CBF1, CBF2, CBF3, and CBF-targeted genes was much lower in Cvi-1 than in Ws-2 plants, and the low-temperature metabolome of Cvi-1 plants was depleted in metabolites affected by CBF3 overexpression. Taken together, the results indicate that the metabolome of Arabidopsis is extensively reconfigured in response to low temperature, and that the CBF cold response pathway has a prominent role in this process.
National Acad Sciences
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