A sandwich enzyme immunoassay for pulmonary surfactant protein D and measurement of its blood levels in drowning victims

S Kamada, Y Seo, K Takahama - Forensic science international, 2000 - Elsevier
A sensitive sandwich enzyme immunoassay for human pulmonary surfactant protein D (SP-
D) was developed and used to examine the blood SP-D levels of drowning victims. Human
SP-D was purified from amniotic fluid by chromatographic methods, and an antibody against
human SP-D was prepared. A polystyrene ball coated with anti-SP-D IgG was incubated
with purified human SP-D, and then with anti-SP-D Fab′-peroxidase conjugate. Peroxidase
activity bound to the polystyrene ball was assayed by fluorometry using 3-(4-hydroxyphenyl) …

[引用][C] A sandwich enzyme immunoassay for pulmonary surfactant protein D and measurement of its blood levels in drowning victims

鎌田秀一 - 2002 - ci.nii.ac.jp
CiNii 博士論文 - A sandwich enzyme immunoassay for pulmonary surfactant protein D and
measurement of its blood levels in drowning victims … A sandwich enzyme immunoassay for
pulmonary surfactant protein D and measurement of its blood levels in drowning victims
サーファクタントプロテインDを用いた溺死診断法に関する研究 … A sandwich enzyme immunoassay
for pulmonary surfactant protein D and measurement of its blood levels in drowning victims
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