A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous …
T Iwaki, FJ Castellino - Cytotechnology, 2008 - Springer
Abstract The Drosophila Schneider S2 (S2) Expression System enables expression of
recombinant proteins constitutively, as well as inductively. This system can establish both
transient and stable transformants with various selection markers. The generation of stable
cell lines for increased expression or large scale expression of the desired protein is
currently accomplished by cotransfection of both the expression and selection vectors. The
selection vectors, pCoHYGRO and pCoBLAST, are commercially available using …
recombinant proteins constitutively, as well as inductively. This system can establish both
transient and stable transformants with various selection markers. The generation of stable
cell lines for increased expression or large scale expression of the desired protein is
currently accomplished by cotransfection of both the expression and selection vectors. The
selection vectors, pCoHYGRO and pCoBLAST, are commercially available using …
A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible …
T Iwaki, K Umemura - Cytotechnology, 2011 - Springer
Although there are several inducible expression systems for mammalian cells, the most
reliable one is the tetracycline-regulated expression system. This system is well-established
and widely used by many researchers. Although Clontech provides several types of cells
that stably express reverse tetracycline transactivator (rtTA), the cells that are not provided
can be generated with pTet-On-Advanced by first integrating this plasmid into the require
type of cell and then introducing the genes of interest. These processes are experimental …
reliable one is the tetracycline-regulated expression system. This system is well-established
and widely used by many researchers. Although Clontech provides several types of cells
that stably express reverse tetracycline transactivator (rtTA), the cells that are not provided
can be generated with pTet-On-Advanced by first integrating this plasmid into the require
type of cell and then introducing the genes of interest. These processes are experimental …
