[HTML][HTML] A single-step affinity column for purification of serotype-5 based adeno-associated viral vectors

A Auricchio, E O'connor, M Hildinger, JM Wilson - Molecular Therapy, 2001 - cell.com
A Auricchio, E O'connor, M Hildinger, JM Wilson
Molecular Therapy, 2001cell.com
Here we describe a single-step affinity column for purification of vectors based on adeno-
associated virus type 5 (AAV5). A sialic-acid-rich protein called mucin was covalently
attached to Sepharose and was found to bind AAV5 vectors. Elution with high salt efficiently
recovered highly active vectors of greater purity than what is achieved with CsCl 2
sedimentation.
Abstract
Here we describe a single-step affinity column for purification of vectors based on adeno-associated virus type 5 (AAV5). A sialic-acid-rich protein called mucin was covalently attached to Sepharose and was found to bind AAV5 vectors. Elution with high salt efficiently recovered highly active vectors of greater purity than what is achieved with CsCl2 sedimentation.
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