Aquaporins in Saccharomyces cerevisiae wine yeast
JE Karpel, LF Bisson - FEMS microbiology letters, 2006 - academic.oup.com
JE Karpel, LF Bisson
FEMS microbiology letters, 2006•academic.oup.comAQY1 and AQY2 were sequenced from five commercial and five native wine yeasts. Of
these, two AQY1 alleles from UCD 522 and UCD 932 were identified that encoded three or
four amino-acid changes, respectively, compared with the Σ1278b sequence. Oocytes
expressing these AQY1 alleles individually exhibited increased water permeability vs. water-
injected oocytes, whereas oocytes expressing the AQY2 allele from UCD 932 did not show
an increase, as expected, owing to an 11 bp deletion. Wine strains lacking Aqy1p did not …
these, two AQY1 alleles from UCD 522 and UCD 932 were identified that encoded three or
four amino-acid changes, respectively, compared with the Σ1278b sequence. Oocytes
expressing these AQY1 alleles individually exhibited increased water permeability vs. water-
injected oocytes, whereas oocytes expressing the AQY2 allele from UCD 932 did not show
an increase, as expected, owing to an 11 bp deletion. Wine strains lacking Aqy1p did not …
Abstract
AQY1 and AQY2 were sequenced from five commercial and five native wine yeasts. Of these, two AQY1 alleles from UCD 522 and UCD 932 were identified that encoded three or four amino-acid changes, respectively, compared with the Σ1278b sequence. Oocytes expressing these AQY1 alleles individually exhibited increased water permeability vs. water-injected oocytes, whereas oocytes expressing the AQY2 allele from UCD 932 did not show an increase, as expected, owing to an 11 bp deletion. Wine strains lacking Aqy1p did not show a decrease in spore fitness or enological aptitude under stressful conditions, limited nitrogen, or increased temperature. The exact role of aquaporins in wine yeasts remains unclear.
Oxford University Press
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