Artificial control of nitrate respiration through the lac promoter permits the assessment of oxygen-mediated posttranslational regulation of the nar operon in …
CE Noriega, V Sharma, JJ Rowe - Journal of bacteriology, 2007 - Am Soc Microbiol
CE Noriega, V Sharma, JJ Rowe
Journal of bacteriology, 2007•Am Soc MicrobiolIn this study, oxygen and nitrate regulation of transcription and subsequent protein
expression of the unique narK1K2GHJI respiratory operon of Pseudomonas aeruginosa
were investigated. Under the control of PLAC, P. aeruginosa was able to transcribe nar and
subsequently express methyl viologen-linked nitrate reductase activity under aerobic
conditions without nitrate. Modulation of PLAC through the LacI repressor enabled us to
assess both transcriptional and posttranslational regulation by oxygen during physiological …
expression of the unique narK1K2GHJI respiratory operon of Pseudomonas aeruginosa
were investigated. Under the control of PLAC, P. aeruginosa was able to transcribe nar and
subsequently express methyl viologen-linked nitrate reductase activity under aerobic
conditions without nitrate. Modulation of PLAC through the LacI repressor enabled us to
assess both transcriptional and posttranslational regulation by oxygen during physiological …
Abstract
In this study, oxygen and nitrate regulation of transcription and subsequent protein expression of the unique narK1K2GHJI respiratory operon of Pseudomonas aeruginosa were investigated. Under the control of PLAC, P. aeruginosa was able to transcribe nar and subsequently express methyl viologen-linked nitrate reductase activity under aerobic conditions without nitrate. Modulation of PLAC through the LacI repressor enabled us to assess both transcriptional and posttranslational regulation by oxygen during physiological whole-cell nitrate reduction.
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