Biolistic co-transformation of Metarhizium anisopliae var. acridum strain CG423 with green fluorescent protein and resistance to glufosinate ammonium
PW Inglis, FJL Aragao, H Frazao… - FEMS microbiology …, 2000 - academic.oup.com
Metarhizium anisopliae var. acridum (syn. M. flavoviride) is recognized as a highly specific
and virulent mycopathogen of locusts and grasshoppers and is currently being developed
as a biological control agent for this group of insects in Brazil. Intact conidia of M. anisopliae
var. acridum strain CG423 were transformed using microparticle bombardment. Plasmids
used were:(1) pBARKS1 carrying the bar gene of Streptomyces hygroscopicus fused to the
Aspergillus nidulans trpC promoter, encoding resistance to glufosinate ammonium (or …
and virulent mycopathogen of locusts and grasshoppers and is currently being developed
as a biological control agent for this group of insects in Brazil. Intact conidia of M. anisopliae
var. acridum strain CG423 were transformed using microparticle bombardment. Plasmids
used were:(1) pBARKS1 carrying the bar gene of Streptomyces hygroscopicus fused to the
Aspergillus nidulans trpC promoter, encoding resistance to glufosinate ammonium (or …
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