Secondary expansion and/or evolution of aggressive subclones are associated with the
disease progression and resistance to chemotherapy in neuroblastoma, and it is important
to track the clonal changes during the treatment period. Cell-free (cf) DNA analysis, namely
liquid biopsy, can detect the genomic change of tumor cells without surgical procedures. In
this report, we showed that serial polymerase chain reaction–based cf DNA neuroblastoma
proto-oncogene quantification is sensitive enough to evaluate the aggressive cellular …