Chemical and posttranslational modification of Escherichia coli acyl carrier protein for preparation of dansyl-acyl carrier proteins
JA Haas, MA Frederick, BG Fox - Protein Expression and Purification, 2000 - Elsevier
Escherichia coli acyl carrier protein (ACP) contains a single tyrosine residue at position 71.
The combined o-nitration of apo-ACP Y71 by tetranitromethane and reduction to 3-
aminotyrosyl-apo-ACP were performed to introduce a specific site for attachment of a dansyl
fluorescent label. Conditions for purification and characterization of dansylaminotyrosyl-apo-
ACP are reported. Dansylaminotyrosyl-apo-ACP was enzymatically
phosphopantetheinylated and acylated in vitro with an overall∼ 30% yield of purified …
The combined o-nitration of apo-ACP Y71 by tetranitromethane and reduction to 3-
aminotyrosyl-apo-ACP were performed to introduce a specific site for attachment of a dansyl
fluorescent label. Conditions for purification and characterization of dansylaminotyrosyl-apo-
ACP are reported. Dansylaminotyrosyl-apo-ACP was enzymatically
phosphopantetheinylated and acylated in vitro with an overall∼ 30% yield of purified …
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