[PDF][PDF] Clinical significance of anti-desmoglein-1 and-3 circulating autoantibodies in pemphigus patients measured by area index and intensity score

A Patsatsi, A KyRIAKOU, A Giannakou… - Acta dermato …, 2014 - researchgate.net
A Patsatsi, A KyRIAKOU, A Giannakou, A Pavlitou-Tsiontsi, A Lambropoulos, D Sotiriadis
Acta dermato-venereologica, 2014researchgate.net
MATERIALS AND METHODS A total of 35 pemphigus patients were consecutively selected
to participate in this longitudinal study. For all patients, diagnosis was confirmed by
histology, direct and indirect immunofluorescence, as well as by detection of circulating anti-
DSG-1 and anti-DSG-3 autoantibodies. ELISA was performed using MBL kits (Nagoya,
Japan). Patients with paraneoplastic pemphigus or pemphigus herpetiformis were excluded.
Titres of circulating autoantibodies were measured at 3 different time-points: baseline (the …
MATERIALS AND METHODS
A total of 35 pemphigus patients were consecutively selected to participate in this longitudinal study. For all patients, diagnosis was confirmed by histology, direct and indirect immunofluorescence, as well as by detection of circulating anti-DSG-1 and anti-DSG-3 autoantibodies. ELISA was performed using MBL kits (Nagoya, Japan). Patients with paraneoplastic pemphigus or pemphigus herpetiformis were excluded. Titres of circulating autoantibodies were measured at 3 different time-points: baseline (the time of initial diagnosis), month 6, and month 12. At the same time-points, the clinical picture was evaluated using both ABSIS and PDAI, total and location-dependent scores (Table I). Location-dependent scores were the ABSIS score for skin involvement, mucosal extent score, mucosal severity score, PDAI score for cutaneous lesions and PDAI-mucous membranes score. PDAI score for cutaneous lesions was defined as the sum of PDAI-skin score and PDAI-scalp score. Both
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