Construction and characterization of a large-fragment chicken bacterial artificial chromosome library
R Zimmer, AMV Gibbins - Genomics, 1997 - Elsevier
A large-insert bacterial artificial chromosome (BAC) library has been constructed from male
chicken genomic DNA using the new pBeloBAC11 vector. The library was prepared in two
parts, such that two-thirds of the BAC library (2976 clones) had an average insert size of 490
kb (80 clones analyzed), after optimization of transformation and HMW DNA size-selection
conditions. Fragments of increased average size were cloned by coupling a second size
strategy using pulsed-field gel electrophoresis with optimized electroporation that favored …
chicken genomic DNA using the new pBeloBAC11 vector. The library was prepared in two
parts, such that two-thirds of the BAC library (2976 clones) had an average insert size of 490
kb (80 clones analyzed), after optimization of transformation and HMW DNA size-selection
conditions. Fragments of increased average size were cloned by coupling a second size
strategy using pulsed-field gel electrophoresis with optimized electroporation that favored …
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