[HTML][HTML] Cryopreserved bone marrow aspirate concentrate as a cell source for the colony-forming unit fibroblast assay

DR Berger, ET Aune, CJ Centeno, NJ Steinmetz - Cytotherapy, 2020 - Elsevier
Purpose The prevalence of connective tissue progenitor cells within a cell-based therapy is
often quantified using the colony-forming unit fibroblast (CFU-F) assay. The present study
investigates the feasibility of using cryopreserved bone marrow aspirate concentrate
(BMAC) as an alternative cell source to fresh BMAC for CFU-F quantification. Methods
Freshly prepared and corresponding cryopreserved BMAC samples from patients receiving
autologous cell therapy (n= 98) were analyzed using the CFU-F assay for comparison …
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