Cytotoxic effects of aflatoxin B1 and its association with cellular components ni chicken embryo primary cultured cells
M Iwaki, T Kitagawa, Y Akamatsu, K Aibara - Biochimica et Biophysica Acta …, 1990 - Elsevier
We have examined the cytotoxicity and cellular incorporation of aflatoxin B 1 (AFB 1) in
several types of established and primary cultured cells. The inhibition of DNA synthesis by
AFB 1 at 1 μg/ml was about 0–30% in the established cell lines, including human hepatic
cells. In chicken primary hepatocytes, however, DNA synthesis as well as RNA and protein
syntheses were strongly inhibited by much lower concentrations of AFB 1, eg, 0.1 μg/ml. In
contrast, chicken primary fibroblasts showed almost no significant response to the toxin …
several types of established and primary cultured cells. The inhibition of DNA synthesis by
AFB 1 at 1 μg/ml was about 0–30% in the established cell lines, including human hepatic
cells. In chicken primary hepatocytes, however, DNA synthesis as well as RNA and protein
syntheses were strongly inhibited by much lower concentrations of AFB 1, eg, 0.1 μg/ml. In
contrast, chicken primary fibroblasts showed almost no significant response to the toxin …
