Canine distemper virus (CDV) is the cause of a severe and highly contagious disease in
dogs. Practical diagnosis of canine distemper based on clinical signs and laboratory tests
are required to confirm CDV infection. The present study aimed to develop a molecular
assay to detect and differentiate field and vaccine CDV strains. Reverse transcription
followed by nested real time polymerase chain reaction (RT-nqPCR) was developed, which
exhibited analytical specificity (all the samples from healthy dogs and other canine infectious …