Detection of dengue viral RNA in mosquito vectors by mixed phase and solution hybridization
KE Olson, CD Blair, BJ Beaty - Molecular and cellular probes, 1990 - Elsevier
A mixed phase hybridization technique was developed to detect dengue virus type 2 (DEN-
2) RNA in pools of infected Aedes albopictus mosquitoes using radiolabelled RNA probes.
This technique used a guanidine thiocyanate extraction procedure to simplify analyte
preparation. The probes contained sequences complementary to portions of the NS-1 or NS-
5 genes of the DEN-2 viral genome. One infected mosquito in a pool of 25 could be detected
in approximately 48 h. RNAs from DEN serotypes 1–4 were extracted from cultured …
2) RNA in pools of infected Aedes albopictus mosquitoes using radiolabelled RNA probes.
This technique used a guanidine thiocyanate extraction procedure to simplify analyte
preparation. The probes contained sequences complementary to portions of the NS-1 or NS-
5 genes of the DEN-2 viral genome. One infected mosquito in a pool of 25 could be detected
in approximately 48 h. RNAs from DEN serotypes 1–4 were extracted from cultured …
以上显示的是最相近的搜索结果。 查看全部搜索结果