Development of a Gene Knockout System in Candida parapsilosis Reveals a Conserved Role for BCR1 in Biofilm Formation
Candida parapsilosis is an important cause of candidiasis, yet few molecular tools are
available. We adapted a recyclable nourseothricin resistance marker gene (SAT1) originally
developed for use with C. albicans in order to generate gene knockouts from C. parapsilosis.
We first replaced the promoters driving expression of the FLP recombinase and the SAT1
genes with the equivalent sequences from C. parapsilosis. We then used the cassette to
generate a homozygous knockout of C. parapsilosis URA3. The ura3 knockouts have …
available. We adapted a recyclable nourseothricin resistance marker gene (SAT1) originally
developed for use with C. albicans in order to generate gene knockouts from C. parapsilosis.
We first replaced the promoters driving expression of the FLP recombinase and the SAT1
genes with the equivalent sequences from C. parapsilosis. We then used the cassette to
generate a homozygous knockout of C. parapsilosis URA3. The ura3 knockouts have …
Development of a gene knockout system in Candida parapsilosis reveals a conserved role for BCR1 in biofilm formation.
DC Ding Chen, G Butler - 2007 - cabidigitallibrary.org
Candida parapsilosis is an important cause of candidosis, yet few molecular tools are
available. We adapted a recyclable nourseothricin resistance marker gene (SAT1) originally
developed for use with C. albicans in order to generate gene knockouts from C. parapsilosis.
We first replaced the promoters driving expression of the FLP recombinase and the SAT1
genes with the equivalent sequences from C. parapsilosis. We then used the cassette to
generate a homozygous knockout of C. parapsilosis URA3. The ura3 knockouts have …
available. We adapted a recyclable nourseothricin resistance marker gene (SAT1) originally
developed for use with C. albicans in order to generate gene knockouts from C. parapsilosis.
We first replaced the promoters driving expression of the FLP recombinase and the SAT1
genes with the equivalent sequences from C. parapsilosis. We then used the cassette to
generate a homozygous knockout of C. parapsilosis URA3. The ura3 knockouts have …
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