Development of rapid, sensitive one-tube duplex RT-PCR assay for specific and differential diagnosis of Chikungunya and dengue
K Saha, R Firdaus, S Chakrabarti… - Journal of virological …, 2013 - Elsevier
Chikungunya and dengue, two arboviral infections are common in South-East Asia and their
early clinical manifestations are very similar hence it is important to discriminate between
them as early as possible for better clinical management. The aim of this study was to design
a rapid, sensitive and specific method for the differential diagnosis of these two viruses
simultaneously. A rapid one-tube duplex RT-PCR assay was developed that requires 110
min including RNA extraction, RT-PCR and agarose gel electrophoresis by using a novel …
early clinical manifestations are very similar hence it is important to discriminate between
them as early as possible for better clinical management. The aim of this study was to design
a rapid, sensitive and specific method for the differential diagnosis of these two viruses
simultaneously. A rapid one-tube duplex RT-PCR assay was developed that requires 110
min including RNA extraction, RT-PCR and agarose gel electrophoresis by using a novel …
Development of rapid, sensitive one-tube duplex RT-PCR assay for specific and differential diagnosis of Chikungunya and dengue.
KS Kallol Saha, RF Rushna Firdaus… - 2013 - cabidigitallibrary.org
Chikungunya and dengue, two arboviral infections are common in South-East Asia and their
early clinical manifestations are very similar hence it is important to discriminate between
them as early as possible for better clinical management. The aim of this study was to design
a rapid, sensitive and specific method for the differential diagnosis of these two viruses
simultaneously. A rapid one-tube duplex RT-PCR assay was developed that requires 110
min including RNA extraction, RT-PCR and agarose gel electrophoresis by using a novel …
early clinical manifestations are very similar hence it is important to discriminate between
them as early as possible for better clinical management. The aim of this study was to design
a rapid, sensitive and specific method for the differential diagnosis of these two viruses
simultaneously. A rapid one-tube duplex RT-PCR assay was developed that requires 110
min including RNA extraction, RT-PCR and agarose gel electrophoresis by using a novel …
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